[Histonet] Decalcification

Sherwood, Margaret MSHERWOOD <@t> PARTNERS.ORG
Wed Dec 8 12:40:36 CST 2010

We have small arteries that were FFPE and sectioned that should have been
decacified before processing.  (The investigator did not indicate as such when
submitting them).  Cutting was extremely difficult and the sections, as you can
imagine, had terrible knife marks and chatter.  Of course, they are important
(for a paper)!  

Is there any way tissue, that has been embedded, can be decalcified at this
point?  Or some other way to treat the block so that we can get better sections?

Thanks for all your help.


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 

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