[Histonet] Reference book for fixatives and their properties

Rocky Parker rparker <@t> monell.org
Tue Dec 7 13:23:02 CST 2010

Hi everyone,

I seem to always need information on different fixatives (half-lives,
storage, usage, physical properties, alternatives, etc.), and I wanted
to know if any of you use a "bible" of sorts in your lab(s). I have a
running tally in my head of problems I've had, things to avoid, etc.
over the years. However, I don't have a good, physical resource for
use in the lab. For instance, we recently had an issue with fixative
mixtures and tissue distortion in addition to some shelf life
questions that I'm assuming (?) would be covered in the type of
reference I'm trying to find.

If you know of a reference right off the top of your head that would
have all of this info (and perhaps mention of sectioning techniques
for TEM, LM, etc.), I would really appreciate that info. I want a book
for my own reference, but I also want to purchase the same resource
for our lab.


Postdoctoral Fellow
Monell Chemical Senses Center
3500 Market St.
Philadelphia, PA  19104

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