[Histonet] delaying Hematoxylin & Eosin staining?

Teresa Iglesias tliglesias <@t> ucdavis.edu
Tue Aug 31 20:54:13 CDT 2010

Thanks, Andrea! Is it imperative to rehydrate in the fixative or can I just
go straight to rehydration in water? Is rehydrating in fixative because of
the long dry-time because I don't see it in any protocols?
Thanks again!
University of CA, Davis

On Tue, Aug 31, 2010 at 8:30 PM, <andreahooper <@t> rocketmail.com> wrote:

> H+E staining should be unaffected by storage of frozen sections at RT. I
> have done this for at least one week and its not a problem. It is IHC or
> enzymatic stains which could pose a problem - depending on fixative etc.
> I do not suggest you rinse in PBS before airdrying, however. I take off
> cryostat directly onto slide and air dry. Air dry cryosections in OCT and
> leave at RT. When ready to stain, just rehydrate in your fixative for 5-10
> min then PBS or water and proceed.
> Sent from my Verizon Wireless BlackBerry
> -----Original Message-----
> From: Teresa Iglesias <tliglesias <@t> ucdavis.edu>
> Sender: histonet-bounces <@t> lists.utsouthwestern.edu
> Date: Tue, 31 Aug 2010 19:38:32
> To: <histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] delaying Hematoxylin & Eosin staining?
> I'm a guest in a lab with very little freezer/fridge space so I'm wondering
> if I can mount fixed-frozen testes tissue onto slides, and once dry, store
> in a slide box at room temperature for a few weeks until all my slides are
> ready for staining.
> Tissues are fixed in 10% formalin then sunk in 20% sucrose. I'll embed in
> OCT (optimal cutting temperature compound) before cryosectioning. I will
> mount them after a rinse in PBS (phosphate buffered solution).
> I worry about the tissues over drying since they are not embedded in
> paraffin. Does anyone have any experience with this?
> Thanks!
> --
> Teresa Iglesias
> Graduate Group in Animal Behavior
> University of California-Davis
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Teresa Iglesias
Graduate Group in Animal Behavior
Department of Evolution and Ecology

University of California-Davis
One Shields Avenue
2320 Storer Hall
Davis, CA 95616
Office: 530-754-7837
Fax: 530-752-1449
tliglesias <@t> ucdavis.edu

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