[Histonet] IHC Control Questions

[Sebree Linda A]

Hi Tobias,

We precut control slides, picking up the sections by dipping the top end
of the slide only into the water bath, air-dry and freeze at -20 for
those antibodies that target labile antigens (the Histonet archives
should help you determine which ones).  If that's not the case,
frequently used control slides are stored at RT.  We've successfully
stored control slides at - 20 for several years with little or no
diminishment of antigenicity.  When we have a case to cut, we remove the
control slide(s) from the freezer, let come to RT, then pick up our
patient section at the middle/bottom of the slide.  We obtain our
control tissue from patient cases.

If in doubt about the stability of your target antigens, and you have
the room, freeze your precut control slides.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victor
Tobias
Sent: Tuesday, August 31, 2010 12:40 PM
To: Histonet
Subject: [Histonet] IHC Control Questions


  I am writing on behalf of our supervisor.

For those of you that place the control tissue on the same slide as the
patient, do you precut the control blocks or cut them at the same time
as the patient? If you precut, how are you storing the slides and how
long can they be stored?

We will be starting off with a control block with 3 tumors in it. Are
you acquiring your control material from positive patient cases or are
you purchasing your control blocks?

I think we are going to need to move to either a sausage roll or micro
array. I believe they are putting the final numbers together and we are
getting a Bond.

Thanks for any feedback
Victor

--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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