[Histonet] Cleaning molds

Fri Aug 27 10:40:37 CDT 2010

I agree with Joyce. 

Here we have neither cleaned regularly nor used mold release for decades - since the solid state cooling plate came into use.

Raoul Regnault 
Anatomic Pathology 
Kootenay Boundary Regional Hospital 
1200 Hospital Bench 
Trail B.C. 
V1R 4M1 

250-368-3311 ext 2263 
fax 250-364-3457 
raoul.regnault <@t> interiorhealth.ca 

þ Please consider the environment before printing this email. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, August 27, 2010 8:33 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Cleaning molds

If you keep your molds in the hot tray of the embedding center, you never have to clean them... I promise. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Friday, August 27, 2010 09:53
To: chapcl <@t> yahoo.com; Webb, Dorothy L; histonet-bounces <@t> lists.utsouthwestern.edu; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Cleaning molds

yeah, I'd be careful of this method. We used to do this at the U.S. Air Force hospital I used to work at when I was active duty. One time, the fire alarm went off and we had to evacuate the lab. Well, there must have been a real problem because we were out of the lab a long time. By the time we got the "all clear" to go back into the lab, I was the first one back and ran into some firemen. Seems the water boiled out of the pot and the paraffin was smoking up a storm.. No one said anything, but I bet that was why we were out of the lab so long. Now, those of you that have worked in a military or government hospital realize all the freaking paperwork that had to be filled out from that one incident. We were ordered by Safety to develop another method. So, we soak our molds in xylene for a couple of hours, covered of course as not expose the lab to the xylene fumes (that would required addition freaking paperwork) followed by a 100% etoh rinse, followed by air drying.

JTT
----- Original Message -----
From: <chapcl <@t> yahoo.com>
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>;
<histonet-bounces <@t> lists.utsouthwestern.edu>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 26, 2010 11:16 AM
Subject: Re: [Histonet] Cleaning molds

> Back when I was using metal cassette lids and an autotechnicon (so no 
> cleaning in the processor) I would throw all the lids and molds into a 
> pot after embedding. Throw a bit of laboratory detergent in, fill with 
> water, and boil on a hot plate. Once I got to a rolling boil, I would 
> turn the heat off. By the end of the day it was cool.  Peel the 
> paraffin off the top, then rinse the molds.
>
> Worked great!  Didn't even need mold release.
>
> Will
>
> Sent from my Verizon Wireless BlackBerry
>
> -----Original Message-----
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Sender: histonet-bounces <@t> lists.utsouthwestern.edu
> Date: Thu, 26 Aug 2010 10:53:58
> To: 
> 'histonet <@t> lists.utsouthwestern.edu'<histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Cleaning molds
>
> Does anyone in histoland clean their embedding molds in a dishwasher? 
> Otherwise, besides placing in the cleaning cycle of your processor, 
> how do sites clean their molds??  Simple, but plaguing question!!!!!!!  
> Thanks all!
>
> Dorothy Webb
>
>
>
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