[Histonet] shrinkage

[Smith, Allen]

When I wrote my thesis on sweat glands, my experience was that fixation in Helly's fluid ("Zenker-formol") and paraffin embedding caused serious shrinkage: about 10-15% in each dimension.

Allen A. Smith
Professor of Anatomy
Barry University School of Podiatric Medicine

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, August 24, 2010 11:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] shrinkage

I think tissue shrinkage is a fairy tail, told by pathologists at the
grossing. ;) "Sure, it is a little bit large, but it will shrink.."
Too big blocks stay too big while embedding.

**just for smiling**


There's a study by Cecil Fox 1985 (Formaldehyde fixation), who showed that
shrinkage while fixation occurs only with concentrated formaldehyd, perhaps
due to the methanol-part in commercial formalin.

For dehydration, "de-fattation" and infiltration I think, it depends on the
gradationsteps of the solutions, the applied temperature and the
"ingredients" of the tissue. With our standard protocol (13 hours VIP) I
never found excessiv shrinkage.
So, like always, it depends...

Gudrun Lang


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Tench,
Bill
Gesendet: Dienstag, 24. August 2010 17:33
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] shrinkage

I have never seen a study, but when training, we were taught that
fixation and processing could result in up to 5-10% shrinkage.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

[None] made the following annotations
---------------------------------------------------------------------
Confidential E-Mail: This e-mail is intended only for the person or entity
to which it is addressed, and may contain information that is privileged,
confidential, or otherwise protected from disclosure. Dissemination,
distribution, or copying of this e-mail or the information herein by anyone
other than the intended recipient is prohibited. If you have received this
e-mail in error, please notify the sender by reply e-mail, and destroy the
original message and all copies. 
---------------------------------------------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet