[Histonet] shrinkage

[Bryan Hewlett]

Richard,

It depends upon the tissue type, the fixative used, time/temperature/ 
gradation of alcohols, intermediate solvent type/time/temp and the wax 
time/temp.
Careful fixation and processing of liver or spleen, using 4% aqueous 
formaldehyde/ graded alcohols /xylene/ wax at 56-60C, results in mean volume 
changes of 30-40%.
This is discussed in JR Bakers seminal text "Principles of Biological 
Microtechnique" 1958. try to find a copy in your library.

You will also find discussion in the EM literature from the 1960's. I recall 
3-8% for resins.

Regards,

Bryan



----- Original Message ----- 
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, August 24, 2010 11:17 AM
Subject: [Histonet] shrinkage



Anybody aware of the degree of shrinkage in paraffin processed tissues 
and/or GMA processed tissues?, many thanks.

                         Cheers
                                  Richard  Edwards

                                      Leicester University.

                                              Leicester  U.K.

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