bhewlett <@t> cogeco.ca
Tue Aug 24 13:58:12 CDT 2010
It depends upon the tissue type, the fixative used, time/temperature/
gradation of alcohols, intermediate solvent type/time/temp and the wax
Careful fixation and processing of liver or spleen, using 4% aqueous
formaldehyde/ graded alcohols /xylene/ wax at 56-60C, results in mean volume
changes of 30-40%.
This is discussed in JR Bakers seminal text "Principles of Biological
Microtechnique" 1958. try to find a copy in your library.
You will also find discussion in the EM literature from the 1960's. I recall
3-8% for resins.
----- Original Message -----
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, August 24, 2010 11:17 AM
Subject: [Histonet] shrinkage
Anybody aware of the degree of shrinkage in paraffin processed tissues
and/or GMA processed tissues?, many thanks.
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