[Histonet] Re: Cryoprotection

[Mejia, Maria]

Can you be more specific & tell us what DW means & please give us the protocol for the sucrose including
the amount of DW used.  This way we can help you.

Regards

Maria Mejia
Histology Supervisor
Department of Neurosurgery
UCSF
San Francisco, CA
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of vaigunda ragavendran [rags_jeg <@t> yahoo.co.in]
Sent: Friday, August 20, 2010 1:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Cryoprotection

Hii,

If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection..

Please lemme know..

Rags,
Postdoctoral Research Fellow,
Verge Lab,
Department of Anatomy and Cell Biology,
Room No: 5800, Saskatoon City Hospital,
701, Queen Street,
Saskatoon, Saskatchewan S7K 0M7,
Canada.

Phone (Lab): +1 306-655-8714
          (Facsimile): +1 306-655-8709



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