[Histonet] Re: Cryoprotection

Mejia, Maria Maria.Mejia <@t> ucsf.edu
Tue Aug 24 12:31:33 CDT 2010

Can you be more specific & tell us what DW means & please give us the protocol for the sucrose including
the amount of DW used.  This way we can help you.


Maria Mejia
Histology Supervisor
Department of Neurosurgery
San Francisco, CA
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of vaigunda ragavendran [rags_jeg <@t> yahoo.co.in]
Sent: Friday, August 20, 2010 1:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Cryoprotection


If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection..

Please lemme know..

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