[Histonet] protocol for Masson-Goldner trichrome modified by Ulrich

[Jack Ratliff]

What are your histological endpoints? What is it you wish to demonstrate with this stain? Is it Rodent? Canine? Bone? Cartilage? Microtomed sections? Thick/Ground section? W/ or W/O implants? I can help you with the protocol, but I might be able to provide more assistance.

Jack

On Aug 20, 2010, at 2:15 PM, "Michael Mashore" <mmashore <@t> vapop.ucsd.edu> wrote:

> Hello everyone,
> 
> 
> 
> I'm trying to use the Masson-Goldner Trichrome stain, and I came across a
> paper that used one that was modified by Ulrich.  There was no citation on
> the paper, and I wanted more details to their protocol, i.e. how they made
> their solutions.  The paper is titled "Histology and research at the hard
> tissue-implant interface using Technovit 9100 New embedding technique" by
> Elmar Willbold
> <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7GHW-50CVR3J-1&_u
> ser=4429&_coverDate=06%2F25%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_d
> ocanchor=&view=c&_searchStrId=1436969926&_rerunOrigin=google&_acct=C00005960
> 2&_version=1&_urlVersion=0&_userid=4429&md5=ac8acdf1a211881097275a05690a4259
> #cor1>  and Frank Witt.  Does anyone know more details on this stain? We are
> using it on hard tissue embedding, where we used methylmethacrylate.
> 
> 
> 
> Thanks for the help!
> 
> 
> 
> 
> 
> 
> 
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