Fw: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)

Thu Aug 19 19:04:57 CDT 2010

~Kim~ 
~Don't be afraid your life will end, 
be afraid it will never begin~
OU Rocks!!!!

--- On Thu, 8/19/10, Kim Tournear <kimtournear <@t> yahoo.com> wrote:

From: Kim Tournear <kimtournear <@t> yahoo.com>
Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)
To: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Date: Thursday, August 19, 2010, 5:02 PM

Bond Max or Bond III users:

BD Biosciences (clone 24) 1:35 with ER1 for 20 minutes (low pH) 
Biocare (BC/24) 1:50 with ER2 for 20 minutes (high pH)
VBS (clone 1EW) 1:150 with ER2 for 25 minutes (high pH)
VBS (clone 1EW) 1:40 with ER1 for 20 minutes (low pH)
Biocare (clone BC/24) 1:50 with ER2 for 20 minutes (high pH)

These protocols come from various sites using the Bond instrument....
Tonsil is the control of choice.

Hope this helps.....

~Kim~ 
~Don't be afraid your life will end, 
be afraid it will never begin~
OU Rocks!!!!

--- On Thu, 8/19/10, Sebree Linda A <LSebree <@t> uwhealth.org> wrote:

From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)
To: "Taylor, Jean" <jtaylor <@t> meriter.com>, histonet <@t> lists.utsouthwestern.edu, ihcrg <@t> googlegroups.com
Date: Thursday, August 19, 2010, 7:57 AM

Hi Jean,

We use Cell Marque's (via VMS) clone 24.  We have to throw the entire
arsenal at our disposal at these stains but they're acceptable.

Good luck Jean; any questions, you know where we live! 

Linda

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor,
Jean
Sent: Thursday, August 19, 2010 9:45 AM
To: 'histonet <@t> lists.utsouthwestern.edu'; 'ihcrg <@t> googlegroups.com'
Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)

Hi Everyone,

I'm trying to find out what company and clone most labs are using for
PAX-5 (BSAP).

Thanks!

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

-----Original Message-----
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Sent: Wednesday, August 18, 2010 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 81, Issue 23

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Today's Topics:

   1. RE: CD34 positive control (McMahon, Loralee A)
   2. Ventana Renaissance Containers (ricky hachy)
   3. CD34 Control (Silverman, Jeffrey)

----------------------------------------------------------------------

Message: 1
Date: Wed, 18 Aug 2010 12:17:39 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] CD34 positive control
To: Joel Reichensperger <jreichensperger <@t> siumed.edu>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<C27AA2A01CEF31469813089E226F582E02D5A7CAA4 <@t> URMCMS7.urmc-sh.rochester.ed
u>

Content-Type: text/plain; charset="us-ascii"

Just about any tissue should work.   It stains for Hematopoietic
stem/progenitor cells, bone marrow stromal cells, endothelial cells,
embryonic fibroblasts
We use a sausage style control here with about 30 different types of
tissue.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joel
Reichensperger [jreichensperger <@t> siumed.edu]
Sent: Wednesday, August 18, 2010 12:05 PM
To: Histonet
Subject: [Histonet] CD34 positive control

  I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.

Joel

--
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensperger <@t> siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)

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------------------------------

Message: 2
Date: Wed, 18 Aug 2010 16:28:39 +0000
From: ricky hachy <elciba <@t> hotmail.com>
Subject: [Histonet] Ventana Renaissance Containers
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT114-W54AB5F08E7E0CFC97193EDCF9D0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Hello ,

I need 4 good plastic containers, where you fill with
xilene,alcool....... for the VENTANA RENAISSANCE .

Anyone could sell me ?

Regards

Ricky

------------------------------

Message: 3
Date: Wed, 18 Aug 2010 12:30:58 -0400
From: "Silverman, Jeffrey" <JSilverman <@t> NSHS.edu>
Subject: [Histonet] CD34 Control
To: "'jreichensperger <@t> siumed.edu'" <jreichensperger <@t> siumed.edu>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<83F4D81747A7094DAFE3AE87151ECB941CE41442A3 <@t> SYKECHXVS01.nslijhs.net>
Content-Type: text/plain; charset="us-ascii"

Joel,
A section of skin will be a fine CD34 control. All collagenous
connective tissue, including the dermis,  are rich in CD34+ dendritic
interstitial fibroblasts as well as CD34 positive endothelium in the
resident vessels. Actually, in my lab we use a section of fallopian tube
from tubal ligation, they are loaded with the fibroblasts and vessels.

Jeff Silverman

------------------------------

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