[Histonet] RE: PAX-5 - B-cell specific activator protein (BSAP)

McMahon, Loralee A Loralee_Mcmahon <@t> URMC.Rochester.edu
Thu Aug 19 10:09:04 CDT 2010


Biocare antibody.  Using the diluent that they recommend.   High pH retrieval for 20 minutes.  And the Dako kit with the mouse linker.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtaylor <@t> meriter.com]
Sent: Thursday, August 19, 2010 10:44 AM
To: 'histonet <@t> lists.utsouthwestern.edu'; 'ihcrg <@t> googlegroups.com'
Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)

Hi Everyone,

I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP).

Thanks!

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

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Subject: Histonet Digest, Vol 81, Issue 23

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Today's Topics:

   1. RE: CD34 positive control (McMahon, Loralee A)
   2. Ventana Renaissance Containers (ricky hachy)
   3. CD34 Control (Silverman, Jeffrey)


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Message: 1
Date: Wed, 18 Aug 2010 12:17:39 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] CD34 positive control
To: Joel Reichensperger <jreichensperger <@t> siumed.edu>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <C27AA2A01CEF31469813089E226F582E02D5A7CAA4 <@t> URMCMS7.urmc-sh.rochester.edu>

Content-Type: text/plain; charset="us-ascii"

Just about any tissue should work.   It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
We use a sausage style control here with about 30 different types of tissue.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger <@t> siumed.edu]
Sent: Wednesday, August 18, 2010 12:05 PM
To: Histonet
Subject: [Histonet] CD34 positive control

  I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.

Joel

--
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensperger <@t> siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)


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Message: 2
Date: Wed, 18 Aug 2010 16:28:39 +0000
From: ricky hachy <elciba <@t> hotmail.com>
Subject: [Histonet] Ventana Renaissance Containers
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT114-W54AB5F08E7E0CFC97193EDCF9D0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"


Hello ,

I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE .

Anyone could sell me ?

Regards

Ricky

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Message: 3
Date: Wed, 18 Aug 2010 12:30:58 -0400
From: "Silverman, Jeffrey" <JSilverman <@t> NSHS.edu>
Subject: [Histonet] CD34 Control
To: "'jreichensperger <@t> siumed.edu'" <jreichensperger <@t> siumed.edu>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <83F4D81747A7094DAFE3AE87151ECB941CE41442A3 <@t> SYKECHXVS01.nslijhs.net>
Content-Type: text/plain; charset="us-ascii"

Joel,
A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis,  are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels.

Jeff Silverman



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End of Histonet Digest, Vol 81, Issue 23
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