[Histonet] PAP

[Tony Henwood]

This is our method:

1.	Tap water          			dip gently 5-10 times
2.	Harris haematoxylin      		5 minutes
3.	Wash in running water
4.	Differentiate in acid/alcohol           	one dip
5.	Wash in running water
6.	Scott's Blueing Solution		one minute
7.	Wash in running water.
8.	95% Alcohol              		dip gently 5-10 times
9.	Modified  PAP Stain (see below)      		three minutes
10.	Rinse excess dye off in 95% Alcohol              
11.	Absolute Alcohol         		dip gently 5-10 times
12.	Absolute Alcohol         		dip gently 5-10 times
13.	Xylol                    		dip gently 5-10 times
14.	Xylol                    		dip gently 5-10 times
15.	Xylol                    		dip gently 5-10 times
16.	Mount in D.P.X. Mounting Medium.

This modification uses a single solution, omitting the OG6 solution and
replacing the light green with the more fade resistant Fast Green FCF.

Modified PAP stain

Stock solutions: 
Prepare 10% solutions of each of the stains as follows:
2.5g Eosin Y (CI 45380) in 25ml distilled water 
1g Fast Green FCF (CI 42053) in 10ml distilled water 
      
      Working Solution:
Mix (for 400ml stain)
12ml Eosin Y stock 
2.5ml Fast Green FCF Stock

Make mixture up to 400ml with 95% alcohol.
Add:
0.8g Phosphotungstic acid
4 drops saturated lithium carbonate.

Mix well. 
Store solution in dark brown, tightly capped bottle.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
Raj
Sent: Thursday, 12 August 2010 10:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PAP



Dear friends,

               does anyone have rapid PAP(cytostain) preparation,for
rapid PAP staining.

 

Aazathraj.P

Technical Officer

Department of Histopathology and Cytology

Apollo Hospitals chennai

India
 
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