[Histonet] CF0145EBF1EB4C4E82768D82886A0C9B8FB06A@PLUTO.ad.murdoch.edu.au

Joe Nocito jnocito <@t> satx.rr.com
Fri Aug 13 17:36:27 CDT 2010

Happy Friday all,
try 10% sodium hydroxide for 1 hour prior to processing. It is important to 
let the slides drain really well before placing them in the oven. You want 
to make sure there is no water between the section and the slide. We use 
Plus slides with plain distilled water in our water baths. Hope this helps.
----- Original Message ----- 
From: "Andrew Burgeson" <napoli <@t> siscom.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, August 13, 2010 3:57 PM
Subject: [Histonet] 
CF0145EBF1EB4C4E82768D82886A0C9B8FB06A <@t> PLUTO.ad.murdoch.edu.au

> Joe "the toe" Nocito...are you out there?
> Joe has good ideas about nails. Maybe he will send out his
> procedure again.
> I like using either potassium hydroxide 10-20% or Sodium
> hydroxide 10-20% for softening nail fragments before
> processing.
> Also, keep in mind that soft tissues attached are equally as
> important and sections of nail beds need to be of high
> quality. A melanoma under a nail can be a bad situation.
> Sometimes in addition to PAS or GMS stains for
> onychomycosis, we have done melanin and iron stains for
> areas of pigment or hemmorhagic depositions.
> Joe...are you out there? lol
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