[Histonet] Acceptable way to "bake" sections onto slides?

[Gibson, Philip]

Hi Everyone

 

Just for clarification; our current practice involves a mixture of
placing racks of slides in an oven for 25 minutes, or using the hot area
of an autostainer (we're talking H&E's here, BTW).

Occasionally, with a particularly urgent case us old-timers will place
individual slides directly onto the floor of an oven for 5 mins which
bakes the sections onto the slides very nicely prior to staining. My
theory is that a 60-70 celsius hotplate will perform the same job for
all of our H&E slides.

 

So far, I haven't noticed any particular artefacts, and we don't suffer
from water pooling between section and slide anyway.

 

I'm trying to find both the "leanest" and cheap (hey - this is the NHS!)
solution by consolidating four stainers and three coverslippers into
just the two machines.

 

Maybe I've not looked closely enough at our super-urgent H&E's...

 

Thanks

 

Phil

 

 

 

----------------------------------------

Phil Gibson

Senior Biomedical Scientist

Histopathology Dept

Royal Victoria Infirmary

Newcastle Upon Tyne

NE1 4LP

 

Ext. 24565

Tel. 0191 2824565

 



This email has been processed by SmoothZap - www.smoothwall.net