[Histonet] Acceptable way to "bake" sections onto slides?
Rene J Buesa
rjbuesa <@t> yahoo.com
Fri Aug 13 07:35:50 CDT 2010
The correct answer is "b": if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of "empty nuclei" that will ruin the sections' usefulness.
--- On Fri, 8/13/10, Gibson, Philip <Philip.Gibson <@t> nuth.nhs.uk> wrote:
From: Gibson, Philip <Philip.Gibson <@t> nuth.nhs.uk>
Subject: [Histonet] Acceptable way to "bake" sections onto slides?
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 13, 2010, 8:23 AM
In our "fairly large" histopathology lab we're hoping to consolidate our
autostainers and coverslippers to only two (Sakura) machines.
In order to efficiently do this, we would need to bypass the
autostainer's oven so that multiple racks of slides can be processed
continuously without any 10-15 minute hold-ups. Therefore, we would like
our four separate microtomist teams to place freshly-cut sections on a
hotplate to "bake" for 10 minutes, before being picked up into racks and
transferred directly to xylene on the autostainer.
Do hotplates work well enough to do this? Two conflicting views in my
lab are (a) Yes, this would work in my experience, and (b) No, this
creates artifacts because water trapped underneath the sections boils
and does damage.
Of course, the more conventional approach would be to use ovens, but
loading and unloading an oven before the autostainer is an additional
What do my fellow histonetters think?
Senior Biomedical Scientist
Royal Victoria Infirmary
Newcastle Upon Tyne
Tel. 0191 2824565
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