[Histonet] brain microvessel staining

[Niloofar Rezvani]

Hi,

I appreciate if someone can answer my question. I am going to stain rats' brain microvessels with ER(estrogen receptor) antibody. We know how we can isolate the vessels but this is the first time to stain them.
I have the microvessels in PBS 1X and do you know how I can fix them with paraformaldehyde and mount them on the gel-subbed slides? I did 2 ways: 1- mount the fresh microvessels on the slide and wait overnight to get dried and then fix them in 2% PFA for 30 min and after that washing steps and the rest of IHC protocol or 2- fix the vessels with 2% PFA in the tube for 30 min and then mount them on the slides and wait overnight to get dried.
Please advise me with any idea,
thanks so much,


Dr. Niloofar Rezvani,Ph.D,PharmD
Postdoctoral Fellow
Graduate School of Nursing
Uniformed Services University
Cell:410.615.9078
Fax:301.295.1707
Email: niloofar.rezvani <@t> usuhs.mil


Classification:  UNCLASSIFIED 
Caveats: None



Classification:  UNCLASSIFIED 
Caveats: None