[Histonet] friable or crumbly O.C.T.

[Bruce W Brodersen]

Anyone have an explanation as to why OCT would be friable or crumbly for
sectioning?  Here's how it was used.
Thanks.

"We held the plastic 'tray' with the tissue in the compound just over the
liquid nitro for 30sec-1min, until it was opaque and white (frozen) and
then dipped the tray into the liquid nitro for 20-30sec., placed in small
bags and then into a cooler with dry ice until shipping."


Bruce W. Brodersen, DVM, PhD
University of Nebraska Veterinary Diagnostic Center
1900 N. 42nd Street
Lincoln, NE  68583-0907

voice (402) 472-1434
FAX (402 472-3094