[Histonet] inconsistent staining after quarterly decon

[Kim Tournear]

Hi Carol,
We use (2) benchmarks in our lab but use Amphyl to decon. After decon, we flush with water by "priming" the systems and then we "purge" reaction buffer and ez prep fluid back into the lines. 
 
Purge alcohol thru the liquid coverslip lines to make sure all the water evaporates out of the lines. Before purging the LCS into the lines make sure the LCS container dry completely before refilling. I suggesting rinsing container with 100% alcohol then wait 15-20 minutes before introducing LCS back into the system.
 
I would suggest you cut 20 full coverage tonsil slides (sections from top to bottom) and run all 20 of them with the same antibody to test your staining (CD20 will work nicely). This will tell you if you are getting even coverage from the top of the slide to the bottom and side to side. It might not be the decon procedure, it might be something else.
 
Hope this helps!!
~Kim Tournear ~ HT (ASCP), QIHC (ASCP)

Histology Supervisor
Tucson Medical Center
Tucson,  AZ
~Don't let your life end before it begins~
OU Rocks!!!!

--- On Thu, 4/29/10, Carol Bryant <cbrya <@t> lexclin.com> wrote:


From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: [Histonet] inconsistent staining after quarterly decon
To: "Histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, April 29, 2010, 9:02 AM


Has anyone else ever experienced inconsistent staining on immunos after the quarterly decontamination of their Benchmark?  I think the Lysol IC is still in the lines after numerous water flushes.
Thank you for any input or suggestions.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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