[Histonet] toe nails falling off slides

[Maria Katleba]

HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide!

(1) Cut the slides and place on Plus(+) slide....
(2) shake off excess water
(3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom.
(4) place in hot oven for 5 mins (50-60 degrees)
(5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour
(6) place slides in rack in oven so that the formalin moisture dries...
(7) slide is ready for use

Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of zodiac29 <@t> comcast.net
Sent: Tuesday, April 27, 2010 12:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] toe nails falling off slides

To all,


I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated.


Thanks,


Jenny
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