[Histonet] H&E Stain

[Vanessa Avalos]

I stain derm tissue. We do H&E only using a linear stainer. Two of my docs
seem to say that stain is very eosinophilic & smudgy. Other Two say not
enough Eosin. Of course!!  Followed is my stain protocol.  This week I have
eliminated the Define and slides seem to not look as eosinophilic  and I
have not heard any negative remarks but it may be to soon. Any one have any
suggestions to change???

 

Sub X 

SubX

Sub X

!00% Alc

100% Alc

100& Alc

95% Alc

95% Alc

Water

Hemat

Hemat

Hemat

Water

Water

Define

Water

Blue

Water

95% Alc

Eosin

95% Alc

100% Alc

100% Alc

SubX

Sub X

 

 

V.Avalos

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