[Histonet] Re: paraformaldehyde

[Sherwood, Margaret]

I ditto Teri's suggestion.  In a recent email to you, I mentioned the dangers of
using powdered paraformaldehyde.  I have since stopped using it.

Peggy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson, Teri
Sent: Monday, April 26, 2010 4:24 PM
To: 'margaret.perry <@t> sdstate.edu'
Cc: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Re: paraformaldehyde

Margaret, good luck getting 37 grams of powdered PFA to go into solution. Even
making a 10% solution is difficult, usually requiring heat (60 degrees C) and/or
sodium hydroxide to get it into solution. Once you do, it's going to want to
repolymerize and you'll end up with a real mess. You'd do much better to use
stock 37% formaldehyde you can buy commercially.

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO


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