[Histonet] histotech positions in San Diego area
Tench, Bill
Bill.Tench <@t> pph.org
Mon Apr 26 12:08:08 CDT 2010
Life is uncertain, but........
We may have an opening in the future. You may send resumes directly to
this email address and I will forward them on to the appropriate people.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
-----Original Message-----
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Sent: Monday, April 26, 2010 10:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 77, Issue 32
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Today's Topics:
1. Re: [IHCRG] ER clone 1D5 or SP1 ? (Richard Cartun)
2. Region 1 conference (Amos Brooks)
3. Re: [IHCRG] ER clone 1D5 or SP1 ? (ancillarypath <@t> mac.com)
4. Mysterious artifact on GI biopsies H&E stain (Urim, Lyudmila)
5. paraformaldayhde (Perry, Margaret)
6. RE: Mysterious artifact on GI biopsies H&E stain (Weems, Joyce)
7. anti 8 hydroxyguanosine (Fabrice gankam)
8. RE: Mysterious artifact on GI biopsies H&E stain (Mike Pence)
9. Any Histology openings in San Diego area? (Jill Cox)
10. (no subject) (Urim, Lyudmila)
11. Re: Two antibodies from the same host with tyramide
(Johnson, Teri)
12. RE: (no subject) (Kaye Ryan)
13. RE: (no subject) (Nails, Felton)
14. RE: Best books for the HTL? (Morken, Tim)
15. RE: (no subject) (Jesus Ellin)
16. RE: [IHCRG] ER clone 1D5 or SP1 ? (Van Eyck, Deb)
17. RE: [IHCRG] ER clone 1D5 or SP1 ? (Patsy Ruegg)
----------------------------------------------------------------------
Message: 1
Date: Sun, 25 Apr 2010 15:04:28 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ?
To: "'ihcrg <@t> googlegroups.com'" <ihcrg <@t> googlegroups.com>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>, <JTAYLOR <@t> Meriter.com>
Message-ID: <4BD459FB.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
I have looked at several clones over the years and I prefer clone 6F11.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 4/23/2010 11:17 AM >>>
I'm wondering which clone of ER most labs are using?
Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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------------------------------
Message: 2
Date: Sun, 25 Apr 2010 19:14:49 -0400
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] Region 1 conference
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<y2z582736991004251614m979ddea5yea99650b77ced385 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
I would like to ask if anyone has any photos of the Region 1
conference
to consider emailing them to me. I was going to post some on the Region
1
Conference web page and possibly put some in the Paraffin Press. If you
are
interested, please drop me a line.
Thanks,
Amos
amosbrooks <@t> gmail.com
------------------------------
Message: 3
Date: Sun, 25 Apr 2010 20:36:18 -0400
From: ancillarypath <@t> mac.com
Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ?
To: "ihcrg Group (E-mail)" <ihcrg <@t> googlegroups.com>, histonet
netserver <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0D48EA46-525D-45A6-BFCD-24662D59FA07 <@t> mac.com>
Content-Type: text/plain; charset=us-ascii; format=flowed;
delsp=yes
When we started our lab 3 years ago, we began with SP1 from day 1, so
I don't have any experience with either 1D5 or 6F11 except in my
previous labs. 1D5 is an excellent clone, and seems to be more
specific than SP1 in the work-up of metastatic carcinoma of unknown
primary site, based on the published literature. The advantage of 6F11
is that, for those of us who use the Allred scoring system, it's the
only clone that was clinically validated by Harvey et al. (JCO 1999)
for this purpose. I agree with Rich.
For those who use SP1, it's a very good clone as a predictive marker
in breast cancer. But again, in the setting of metastatic workup, it
is NOT recommended, as it will pick up too many primary lung cancers
and some colon cancers (personal experience).
Hadi
================================
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories
President,
Ancillary Pathways
7000 62nd Avenue, PH-C
Miami, FL 33143
T 305-740-4440
F. 786-513-0175
www.vitromolecular.com
www.ancillarypath.com
On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote:
> I have looked at several clones over the years and I prefer clone
> 6F11.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 4/23/2010 11:17 AM >>>
>
> I'm wondering which clone of ER most labs are using?
>
> Thanks,
> Jean Taylor, HT(ASCP)QIHC
> IHC Tech
> Meriter Health Services
> Madison, WI
>
>
> --
> Subscription settings:
http://groups.google.com/group/ihcrg/subscribe?hl=en
>
------------------------------
Message: 4
Date: Mon, 26 Apr 2010 07:23:57 -0400
From: "Urim, Lyudmila" <lurim <@t> challiance.org>
Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6774152B580A6E44B38487B24A331E7C131603E0 <@t> sasquatch.cphc.local>
Content-Type: text/plain; charset="us-ascii"
> Hi,
>
> We have been getting a similar staining artifact on some GI biopsies
> randomly while the rest GI biopsies look fine.
> Also on the affected GI biopsies there is often only 1 or 2 spots of
> the biopsy that are affected while the rest of the biopsy looks fine.
> The artifact is being reproduced on each cut; also the artifact
> doesn't disappear after the affected biopsies get reprocessed.
> The artifact can be described as a foggy, smudgy stain, with deformed
> nucleus and not enough cell details.
> All our specimens are fixed in 10%NBF.
>
> Please let me know if you have any ideas.
> Lucy
------------------------------
Message: 5
Date: Mon, 26 Apr 2010 07:43:53 -0500
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] paraformaldayhde
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FCA5EF47F9BC694CBB4C58FEA04219634CCFB69F1C <@t> SDSU-MBX.jacks.local>
Content-Type: text/plain; charset="us-ascii"
In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of
powdered paraformaldyhyde for the 37% formalin or do I need to buffer
it?
------------------------------
Message: 6
Date: Mon, 26 Apr 2010 09:48:08 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Mysterious artifact on GI biopsies H&E stain
To: "Urim, Lyudmila" <lurim <@t> challiance.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164015DCCBF11 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
I would check to see what medication the patient was on or if something
was done during the procedure to affect the biopsies..
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Urim,
Lyudmila
Sent: Monday, April 26, 2010 07:24
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain
> Hi,
>
> We have been getting a similar staining artifact on some GI biopsies
> randomly while the rest GI biopsies look fine.
> Also on the affected GI biopsies there is often only 1 or 2 spots of
> the biopsy that are affected while the rest of the biopsy looks fine.
> The artifact is being reproduced on each cut; also the artifact
> doesn't disappear after the affected biopsies get reprocessed.
> The artifact can be described as a foggy, smudgy stain, with deformed
> nucleus and not enough cell details.
> All our specimens are fixed in 10%NBF.
>
> Please let me know if you have any ideas.
> Lucy
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Message: 7
Date: Mon, 26 Apr 2010 09:00:32 -0500
From: Fabrice gankam <gankam <@t> googlemail.com>
Subject: [Histonet] anti 8 hydroxyguanosine
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<u2k86c37d601004260700ufa940a43t440c8d0628263a3 <@t> mail.gmail.com>
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Hey guys was wondering if any of you used the anti 8 hydroxyguanosine to
detect free radical induced DNA and RNa damage.
which antibody is the best. we tried the one from abdserotec and it is a
disaster. the background is just horrible.
any idea ?
2010/4/26 Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
> In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of
> powdered paraformaldyhyde for the 37% formalin or do I need to buffer
it?
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 8
Date: Mon, 26 Apr 2010 09:04:43 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Mysterious artifact on GI biopsies H&E stain
To: "Urim, Lyudmila" <lurim <@t> challiance.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C017A3DDF <@t> is-e2k3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
It could be a "hot biopsy"?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Urim,
Lyudmila
Sent: Monday, April 26, 2010 6:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain
> Hi,
>
> We have been getting a similar staining artifact on some GI biopsies
> randomly while the rest GI biopsies look fine. Also on the affected
> GI biopsies there is often only 1 or 2 spots of the biopsy that are
> affected while the rest of the biopsy looks fine. The artifact is
> being reproduced on each cut; also the artifact doesn't disappear
> after the affected biopsies get reprocessed. The artifact can be
> described as a foggy, smudgy stain, with deformed nucleus and not
> enough cell details. All our specimens are fixed in 10%NBF.
>
> Please let me know if you have any ideas.
> Lucy
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------------------------------
Message: 9
Date: Mon, 26 Apr 2010 07:31:50 -0700 (PDT)
From: Jill Cox <jcox90 <@t> yahoo.com>
Subject: [Histonet] Any Histology openings in San Diego area?
To: "Histonet <@t> Lists. Utsouthwestern. Edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <519989.77668.qm <@t> web56803.mail.re3.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi Netters, I have a friend looking to relocate to San Diego area and is
looking for a Histology position, she is certified HT. Any help would be
greatly appreciated, thanks in advance!! Jill
Jill Cox HT (ASCP)
Arizona Dermatology
4232 E Cactus Rd
Phoenix AZ 85032
------------------------------
Message: 10
Date: Mon, 26 Apr 2010 11:10:08 -0400
From: "Urim, Lyudmila" <lurim <@t> challiance.org>
Subject: [Histonet] (no subject)
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6774152B580A6E44B38487B24A331E7C131603E9 <@t> sasquatch.cphc.local>
Content-Type: text/plain; charset="us-ascii"
Hi,
I am looking into purchasing a microwave tissue processor.
I would be interested to hear from people who has had experience with
microwave tissue processing. And what brands would you recommend?
Thanks a lot,
Lucy
------------------------------
Message: 11
Date: Mon, 26 Apr 2010 10:16:26 -0500
From: "Johnson, Teri" <TJJ <@t> stowers.org>
Subject: [Histonet] Re: Two antibodies from the same host with
tyramide
To: "'anonwums1 <@t> gmail.com'" <anonwums1 <@t> gmail.com>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BD62CBAC4395B94096109020651BE2EC1301F62D15 <@t> EXCHMB-02.stowers-institute.
org>
Content-Type: text/plain; charset="us-ascii"
Adam,
Thanks for the detailed protocol. I can't explain it either but I'll
throw some ideas out there and maybe it'll stimulate discussion.
Hopefully Chris vanderLoos will chime in.
Have you tried doing the labeling in reverse, using the VE-Cadherin
first and then blocking with the goat IgG, and then doing the other
antibody/tyramide protocol? I'm not sure what effect, if any, the
hydrogen peroxide might have on the anti-goat fluorescent 649 label, so
you could certainly do that block prior to doing the first primary
antibody incubation.
Also, you may have to double the concentration of the Cadherin to get it
to label at the same intensity as you do in single staining. But still I
might have expected to see a signal.
Do the two antibodies co-express? Could there be something with the
covalent binding of the HRP-tyramide complex that shelters the antigenic
sites for the second antibody? That's why I wondered what might happen
if you reversed your protocol.
Let us know if you get it figured out and what fixed it for you.
Good luck,
Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO
------------------------------
Message: 12
Date: Mon, 26 Apr 2010 11:25:50 -0400
From: "Kaye Ryan" <kryan <@t> nfderm.com>
Subject: RE: [Histonet] (no subject)
To: "Urim, Lyudmila" <lurim <@t> challiance.org>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DCB07C3EF84D92439E5A453D8D5B5EB004AED5 <@t> exchserver.NFDA.com>
Content-Type: text/plain; charset="us-ascii"
I highly recommend the Milestone microwave tissue processors. I have
used their microwave tissues processors in two different locations and
have found them to be very reliable, user friendly, reproducible and
cost effective. They also have wonderful tech support.
Kaye Ryan
Histology Supervisor
North Florida Dermatology
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Urim,
Lyudmila
Sent: Monday, April 26, 2010 11:10 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi,
I am looking into purchasing a microwave tissue processor.
I would be interested to hear from people who has had experience with
microwave tissue processing. And what brands would you recommend?
Thanks a lot,
Lucy
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Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 13
Date: Mon, 26 Apr 2010 10:49:08 -0500
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: RE: [Histonet] (no subject)
To: "'Urim, Lyudmila'" <lurim <@t> challiance.org>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1FE5960057C084CA389CE97779062904EBD29D2 <@t> TCDMSG01.ad.TexasChildrensHosp
ital.org>
Content-Type: text/plain; charset=us-ascii
It really depends on your volume to be processed and what you are
planning to process.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Urim,
Lyudmila
Sent: Monday, April 26, 2010 10:10 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi,
I am looking into purchasing a microwave tissue processor.
I would be interested to hear from people who has had experience with
microwave tissue processing. And what brands would you recommend?
Thanks a lot,
Lucy
_______________________________________________
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Message: 14
Date: Mon, 26 Apr 2010 08:54:23 -0700
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] Best books for the HTL?
To: "Paula Sicurello" <patpxs <@t> gmail.com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1AAF670737F193429070841C6B2ADD4C01818AC169 <@t> EXMBMCB15.ucsfmedicalcenter.
org>
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Paula,
Carson's book is probably them most relevant to basic histology these
days (Sheehans is still very good for detail on technique and stains but
has a lot of old material that you will rarely, if ever, see anymore).
Bancroft's book is also excellent, has more detail on given stains and
has more on management (QC, etc).
For the HTL I also recommend the ASCP book "Quality Management in
Anatomic Pathology" by Nakhleh and Fitzgibbons.
Plus the usual NSH study guides.
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Sunday, April 25, 2010 7:18 AM
To: HistoNet
Subject: [Histonet] Best books for the HTL?
Hello Listers,
I am going to study for the HTL exam after being in histology for over
20
years. It seems like more and more labs want the certification
and less and less schools are offering the training that's required for
the
HT/HTL.
Anyway, which books are the best to use for the HTL exam? ASCP
mentions
Bancroft an Gamble "Theory and Practice of Histological Technique"
and Garcia "Clinical Laboratory Management".
What do you recently certified HTLs think about those suggestions? I'm
not
getting down on the older certified HTLs but want the opinion of folks
who
went through the grinder recently.
Thanks,
--
Paula Sicurello
6 of 6
Duke University EM Lab
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Message: 15
Date: Mon, 26 Apr 2010 09:02:43 -0700
From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
Subject: RE: [Histonet] (no subject)
To: "Urim, Lyudmila" <lurim <@t> challiance.org>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29BE166A2CF48D459853F8EC57CD37E8021C6277 <@t> EXCHANGECLUSTER.yumaregional.l
ocal>
Content-Type: text/plain; charset="us-ascii"
I would look at this very carefully, especially in light of the new
regulation that were just released by the CAP on ER and PR. But that is
up to each individual lab
Jesus A Ellin HT/PA ASCP
Department of Pathology/Histology
Yuma Regional Medical Center
2400 South Ave A
Yuma, AZ 85364 - 7170
( Office: (928) 336-1743
( Fax: (928) 336-7319
* Email: jellin <@t> yumaregional.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Urim,
Lyudmila
Sent: Monday, April 26, 2010 8:10 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi,
I am looking into purchasing a microwave tissue processor.
I would be interested to hear from people who has had experience with
microwave tissue processing. And what brands would you recommend?
Thanks a lot,
Lucy
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 16
Date: Mon, 26 Apr 2010 11:29:34 -0500
From: "Van Eyck, Deb" <deb.vaneyck <@t> phci.org>
Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?
To: <ancillarypath <@t> mac.com>, "ihcrg Group (E-mail)"
<ihcrg <@t> googlegroups.com>, "histonet netserver"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<37212590E341574B96E6796D27DF42DAB04E95 <@t> RWDEX3.WHS.phci.org>
Content-Type: text/plain; charset="US-ASCII"
This is a great discussion lets also talk about PR clones since the
ASCO/CAP guidelines just came out ------Hadi or Rich I know they only
list two PR clones one is Dako 1294-----what is the other 312? Deb
________________________________
From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf
Of ancillarypath <@t> mac.com
Sent: Sunday, April 25, 2010 7:36 PM
To: ihcrg Group (E-mail); histonet netserver
Subject: Re: [IHCRG] ER clone 1D5 or SP1 ?
When we started our lab 3 years ago, we began with SP1 from day 1, so I
don't have any experience with either 1D5 or 6F11 except in my previous
labs. 1D5 is an excellent clone, and seems to be more specific than SP1
in the work-up of metastatic carcinoma of unknown primary site, based on
the published literature. The advantage of 6F11 is that, for those of us
who use the Allred scoring system, it's the only clone that was
clinically validated by Harvey et al. (JCO 1999) for this purpose. I
agree with Rich.
For those who use SP1, it's a very good clone as a predictive marker in
breast cancer. But again, in the setting of metastatic workup, it is NOT
recommended, as it will pick up too many primary lung cancers and some
colon cancers (personal experience).
Hadi
================================
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories
President,
Ancillary Pathways
7000 62nd Avenue, PH-C
Miami, FL 33143
T 305-740-4440
F. 786-513-0175
www.vitromolecular.com
www.ancillarypath.com
On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote:
I have looked at several clones over the years and I prefer clone 6F11.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
"Taylor, Jean" <jtaylor <@t> meriter.com> 4/23/2010 11:17 AM
>>>
I'm wondering which clone of ER most labs are using?
Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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Message: 17
Date: Mon, 26 Apr 2010 10:46:09 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?
To: <deb.vaneyck <@t> phci.org>, <ancillarypath <@t> mac.com>, "'ihcrg Group
\(E-mail\)'" <ihcrg <@t> googlegroups.com>, "'histonet netserver'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C2A6AC4D0BBF46C580D280D99F2B98F5 <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
Did I understand correctly from Dr. Hammond in Florida that the ER
ASCO/CAP
guidelines extended the fixation time to 72 hours? Are they changing
the
Her2 guidelines to match the ER? If so, has that happened yet? I have
people very anxious to stop having techs work on the weekends to comply
with
the 48 hour fixation limits.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
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From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf
Of
Van Eyck, Deb
Sent: Monday, April 26, 2010 10:30 AM
To: ancillarypath <@t> mac.com; ihcrg Group (E-mail); histonet netserver
Subject: RE: [IHCRG] ER clone 1D5 or SP1 ?
This is a great discussion lets also talk about PR clones since the
ASCO/CAP
guidelines just came out ------Hadi or Rich I know they only list two PR
clones one is Dako 1294-----what is the other 312? Deb
_____
From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf
Of
ancillarypath <@t> mac.com
Sent: Sunday, April 25, 2010 7:36 PM
To: ihcrg Group (E-mail); histonet netserver
Subject: Re: [IHCRG] ER clone 1D5 or SP1 ?
When we started our lab 3 years ago, we began with SP1 from day 1, so I
don't have any experience with either 1D5 or 6F11 except in my previous
labs. 1D5 is an excellent clone, and seems to be more specific than SP1
in
the work-up of metastatic carcinoma of unknown primary site, based on
the
published literature. The advantage of 6F11 is that, for those of us who
use
the Allred scoring system, it's the only clone that was clinically
validated
by Harvey et al. (JCO 1999) for this purpose. I agree with Rich.
For those who use SP1, it's a very good clone as a predictive marker in
breast cancer. But again, in the setting of metastatic workup, it is NOT
recommended, as it will pick up too many primary lung cancers and some
colon
cancers (personal experience).
Hadi
================================
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories
President,
Ancillary Pathways
7000 62nd Avenue, PH-C
Miami, FL 33143
T 305-740-4440
F. 786-513-0175
www.vitromolecular.com
www.ancillarypath.com
On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote:
I have looked at several clones over the years and I prefer clone 6F11.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
"Taylor, Jean" <jtaylor <@t> meriter.com> 4/23/2010 11:17 AM >>>
I'm wondering which clone of ER most labs are using?
Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
--
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