[Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ?

[ancillarypath <@t> mac.com]

When we started our lab 3 years ago, we began with SP1 from day 1, so  
I don't have any experience with either 1D5 or 6F11 except in my  
previous labs. 1D5 is an excellent clone, and seems to be more  
specific than SP1 in the work-up of metastatic carcinoma of unknown  
primary site, based on the published literature. The advantage of 6F11  
is that, for those of us who use the Allred scoring system, it's the  
only clone that was clinically validated by Harvey et al. (JCO 1999)  
for this purpose. I agree with Rich.

For those who use SP1, it's a very good clone as a predictive marker  
in breast cancer. But again, in the setting of metastatic workup, it  
is NOT recommended, as it will pick up too many primary lung cancers  
and some colon cancers (personal experience).

Hadi

================================
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories
President,
Ancillary Pathways
7000 62nd Avenue, PH-C
Miami, FL 33143
T 305-740-4440
F. 786-513-0175
www.vitromolecular.com
www.ancillarypath.com


On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote:

> I have looked at several clones over the years and I prefer clone  
> 6F11.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 4/23/2010 11:17 AM >>>
>
> I'm wondering which clone of ER most labs are using?
>
> Thanks,
> Jean Taylor, HT(ASCP)QIHC
> IHC Tech
> Meriter Health Services
> Madison, WI
>
>
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