[Histonet] new er pr cap guidelines

Fri Apr 23 10:29:31 CDT 2010

In those situations you can set up your processors to hold in formalin until the time limit is reached and then switch to 70%
Sample comes in on Friday.  Place it in formalin (on the processor) until the 48 hours have been reached most likely Sunday afternoon, then switch to an extended 70% alcohol, then continue to process so your cassettes will come off on Monday morning.  It might mean that you have a 6-8 hour 70% step, but it should be ok.  
OF COURSE, run some test tissues first to make sure. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: Mike Pence [mpence <@t> grhs.net]
Sent: Friday, April 23, 2010 10:58 AM
To: McMahon, Loralee A; Patsy Ruegg; Morken, Tim; Maxim Peshkov
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] new er pr cap guidelines

My problem is that we do not work weekends. If I have a breast that comes in on Friday afternoon, I have to cut it in and have it embedded on Sat. morning. If the guidelines are 72 hr for everything then I can process it on Mondays.

-----Original Message-----
From: McMahon, Loralee A [mailto:Loralee_Mcmahon <@t> URMC.Rochester.edu]
Sent: Friday, April 23, 2010 9:22 AM
To: Mike Pence; Patsy Ruegg; Morken, Tim; Maxim Peshkov
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] new er pr cap guidelines

I am not sure what the problem is here.  You have to fix for up to 72 hours no more you can do less time.  If you are already fixing your her2 for 48 hours, then what needs to change?   You are not going to submit different cassettes for Er/Pr to fix for longer?  You will submit them all and fix them all for 48 hours max.  The guideline is to prevent under AND over fixation.   Not to submit two different samples to fix for different times.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence <@t> grhs.net]
Sent: Friday, April 23, 2010 10:16 AM
To: Patsy Ruegg; Morken, Tim; Maxim Peshkov
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] new er pr cap guidelines

This was my first post about this issue: (I got no responses for)

Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours?

I am not sure why CAP would not address this up front. This is a big problem in our lab.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Friday, April 23, 2010 8:26 AM
To: 'Morken, Tim'; 'Maxim Peshkov'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] new er pr cap guidelines

Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I
recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Tim
Sent: Thursday, April 22, 2010 11:06 AM
To: Maxim Peshkov
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] new er pr cap guidelines

Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page:

http://tinyurl.com/34kxhtn

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov
Sent: Thursday, April 22, 2010 9:55 AM
To: anita dudley
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] new er pr cap guidelines

Anita:
Maybe your pathologist mean this recommendations:
"Consensus Recommendations on Estrogen Receptor
Testing in Breast Cancer By Immunohistochemistry"
(Appl Immunohistochem Mol Morphol 2008;16:513-520)?
These recommendations came not from CAP.
It is refer only to ER, but not PR.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.

---Original message---

> Date: Tue, 20 Apr 2010 09:46:43 -0500
> From: anita dudley <azdudley <@t> hotmail.com>
> Subject: [Histonet] new er pr cap guidelines
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <SNT122-W5922BE5AC0A3D838F5B6EBD10A0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> has anyone seen the new er pr guidelines for er and
> pr?  my pathologist just came through all in a tizzy
> about it.  just wondering if anyone else has seen it.
> the fixation times were also suppose to change,  to
> 72 hrs fixation for breast.
>
>
>
> thanks for anybodies input,  anita, providence hosp. mobile alabama

--
С уважением,
 Maxim                          mailto:Maxim_71 <@t> mail.ru

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