[Histonet] protein concentration for Cy2

[James L Burchette]

Eric:

Are you using Cy2 labeled goat anti-mouse IgG (H&L) from Jackson 
ImmunoResearch Labs?
According to their catalog (product number 115-225-003), the stock is 2.0 
mg/ml. At 1:100, your working concentration would be 20 ug/ml.

Jim B




"Breisch, Eric" <ebreisch <@t> rchsd.org> 
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04/22/2010 03:51 PM

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[Histonet] protein concentration for Cy2






This question if for anyone doing C4d immunoflourescence staining. Could
someone share what protein concentration the Cy2 labeled anti-mouse IgG
is prepared at and what buffer is used for that? Our final dilution of
the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original
protein concentration for the dry Cy2 should be. Thank you for your
assistance it is greatly appreciated.
 
 
Eric A. Breisch
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