[Histonet] Frozen Rat Heart

[Merced M Leiker]

Hi Sharon,

1. Stuff the tissue in a cryovial or wrap it in foil and throw it in liquid 
N.

2. Put the tissue in a cryomold with OCT covering it and either place it on 
some frozen isopentane; put it in a slurry of isopentane and dry ice 
pieces; or suspend over liquid N vapors to freeze.

Not sure if this is what you were looking for, precisely?

Regards,
Merced

--On Thursday, April 22, 2010 11:06 AM -0400 "Shaw, Sharon" 
<shshaw <@t> WPI.EDU> wrote:

> Does anybody have a protocol on freezing rat heart that you can share
> with me. Thanks,
> Sharon
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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