[Histonet] Dark background with Gomori's reticulin impregnation

[Maxim Peshkov]

Andre:
1. Red shadows in reticulin maybe as results
too long steps with gold chloride. In your
protocol it is for 5 mins. Try this step as 1 min.
2. Try substitute in step 13) 20% formaline at 10%.
It can be prevent high gray background.
3. Chek pH of DW before nuclear fast red. It must be
approx. 5-5.5. At reduction pH red conterstain will
look as you have seen.
Maxim Peshkov,
Russia,
Taganrog.
                      mailto:Maxim_71 <@t> mail.ru