[Histonet] Re: IHC mouse antibody on mouse tissue
Hobbs, Carl
carl.hobbs <@t> kcl.ac.uk
Thu Apr 15 09:54:05 CDT 2010
Imho....just do it on your tissue and include a no primary control.
If the latter is clear, don't worry about MOM.
If it is not, then we can advise further.
You don't state what Ab/tissue/detection system you are using......
This would help a lot as protocols will vary according to the tissue used .
I never want to use a more complicated detection system than I have to, just because it is MOM.
Occam's razor!
carl
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