[Histonet] IHC on FNA

[Cynthia Pyse]

Linda
I agree with you that is why I asked the question. We do not have a
cryostat, so frozen sections are out of the question. I thought of making
cytospin slides out of positive fluid but then you run into the problem of
any positive cells making it onto the slide. Would any of you run the FNA
slide without pretreatment and the FFPE control with the pretreatment? I
appreciate everyone suggestions.


Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com

  

-----Original Message-----
From: Sebree Linda A [mailto:LSebree <@t> uwhealth.org] 
Sent: Friday, April 09, 2010 12:40 PM
To: Cynthia Pyse; Histonet
Subject: RE: [Histonet] IHC on FNA

This topic touches a nerve with me.  We recently went back to receiving
requests on FNAs after years of not doing any.  When we were routinely
staining them with IHC, we would use a frozen tissue section for a
control as the same protocols generally worked for both, i.e. no HIER,
protease, etc as may be needed for FFPE.  When we stopped doing FNA IHC
routinely, we eliminated our frozen section control inventory.

Now that we're doing them again, it was decided to use FFPE controls and
their accompanying IHC protocols.  I  do not agree with this practice as
I feel there are too many differences between FNA preparations and FFPE
sections.  I was voted down so now we are using HIER, protease, etc.
whatever the FFPE protocol calls for on these FNA preparations.
Surprisingly, at least to me, there are still cells left on the FNA
preps after even the harshest retrieval protocols.

We also don't run negative controls of the FNAs.  The argument being
that there are totally different cells from one slide to the next so a
comparison between a negative control slide and one that received
antibody is useless.

So that's what we're doing even though I don't agree with it.

At the time this all came about, I also queried Histonet as to common
practices so the archives should have some info. also.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia
Pyse
Sent: Friday, April 09, 2010 11:16 AM
To: 'Histonet'
Subject: [Histonet] IHC on FNA

Happy Friday Histonetters

What is everyone using for controls for FNA IHC stains. We routinely
stain human FFPE sections and only have controls for tissue. Recently we
had a few cases when the only sample was an FNA to perform IHC on. Any
suggestions would be welcome .

Regards

 

Cindy Pyse, CLT, HT (ASCP)

Histology Supervisor

X-Cell Laboratories

e-mail cpyse <@t> x-celllab.com

 

 

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