[Histonet] Re: Histonet Digest, Vol 77, Issue 10
Hocking, Mark
mark.hocking <@t> ventana.roche.com
Thu Apr 8 12:29:00 CDT 2010
D
Mark Hocking
Symphony Technical Applications Specialist
Ventana Medical Systems, Inc.
A member of the Roche group
Message sent via blackberry
----- Original Message -----
From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu>
To: histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>
Sent: Thu Apr 08 13:03:49 2010
Subject: Histonet Digest, Vol 77, Issue 10
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Today's Topics:
1. RE: frozen lung sections (Tony Henwood)
2. Re: BREAST IMPLANTS AND HARDWARE (Anne van Binsbergen)
3. Traveling Histo Tech (Marshall, Kimberly)
4. RE: TX Society meeting? (Nita Searcy)
5. trich/bouin's/FS/Inst cleaning/mast cells (John PJ Coleman)
6. eProtocols (Weems, Joyce)
7. Sestrin 2 (Bernard Martin)
8. Re: eProtocols (DKBoyd <@t> chs.net)
9. RE: Traveling Histo Tech (Cheri Miller)
10. RE: Travelling Histo Tech (Edwards, Richard E.)
11. <no subject> (Thotakura, Anil Kumar)
12. CD 25 protocol on Benchmark (Gudrun Lang)
13. CD138 IHC (julia hough)
14. Re: eProtocols (Bill)
15. Re: Just three months????? (Michael LaFriniere)
----------------------------------------------------------------------
Message: 1
Date: Thu, 8 Apr 2010 11:16:31 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] frozen lung sections
To: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC55520685392F <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Betsy,
I would dissolve the OCT in Hanks solution (or similar cell culture
medium - not saline). Place in dilute OCT (50/50 with distilled water),
place on stirrer until the lung settles in the OCT 30-60min. Blot with
absorbent paper, refreeze in fresh OCT. It should cut quite well.
I have used this previously on some tissue that was received the same
way. It seems that the OCT had "freeze-dried" prior to receipt in my
lab.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Molinari, Betsy
Sent: Thursday, 8 April 2010 12:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] frozen lung sections
Hi all,
I have received some lung samples on OCT. These lungs have not been in
PBS or any fixative. They went directly from dissection into OCT and
frozen.
I cannot get a section. The temp of the cryostat is -20. I have done
some reading and it seems the best results come from immersion in PBS,
then 4% paraformaldehyde then into sucrose. Can anyone clarify the
procedure or offer advice? I am unfamiliar with this technique since
most of my frozen sectioning is heart tissue and it does not present the
same problem as the lung.
Thanks,
Betsy
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812
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Message: 2
Date: Thu, 8 Apr 2010 08:11:50 +0400
From: Anne van Binsbergen <annigyg <@t> gmail.com>
Subject: Re: [Histonet] BREAST IMPLANTS AND HARDWARE
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<i2vf8332fbe1004072111q93570a6azebd77b608880ab61 <@t> mail.gmail.com>
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your path dept is being 'used' as a tracking system for these items - the
charge is perfectly justifiable - we do it all the time
tell the plastics guys to set up their own tracking system for these samples
- less hassle for the lab!!!
everything removed from a patient comes to my lab - except forskins from
infants and certain debridements
we have many 88300 charges
regarding the release to be signed - from a medico-legal point of view it
would be best to get someone to sign and get a copy of SS number (or some
sort of ID) just in case
my 2 cents worth
AbuDhabiAnnie
On 7 April 2010 22:17, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:
> Our pathology group charges a "gross only" charge for hardware and implants
> removed from surgery that go to the pathology department. Our current policy
> states that normally these devices do not have to be sent to pathology
> however when the material is requested by the patient to be returned to the
> patient or the patient's clinician it must go to the pathology department
> for accessioning and documentation. Since we do a gross description and
> work with the proper paperwork for release of the items we currently charge
> an 88300 charge which is a gross only charge. Our plastics department
> questions the necessity of this position and why we are charging for it.
> Can anybody else share how they are handling these types of materials
> removed at surgery? In addition our risk management team tells us that
> these devices have to have a release signed by the patient before they can
> be given to the patient or the patient's surgeon.
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
>
> ________________________________
> This message (including any attachments) contains confidential information
> intended for a specific individual and purpose, and is protected by law. If
> you are not the intended recipient, you should delete this message. Any
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--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
------------------------------
Message: 3
Date: Thu, 8 Apr 2010 07:02:45 -0400
From: "Marshall, Kimberly" <Kimberly.Marshall <@t> ahss.org>
Subject: [Histonet] Traveling Histo Tech
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<136EE5CD3735EE4BB86FE88F4B6D0172D30C3D08 <@t> LKMVXCHMB22.ADVENTISTCORP.NET>
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Hello all,
A few years back I went on vacation and we used a traveling Histo tech to cover. Back then I thought it was a neat idea and said to myself, when the kids are grown and out of the house I am gonna do that. Well that time has come, so before I started making calls and researching it I wanted to ask my fellow Histo folks what they know about it. Is there still a market for traveling techs? Any information about this would sure be appreciated.
Thanks in advance
Kimberly Marshall HT(ASCP)
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Message: 4
Date: Thu, 08 Apr 2010 06:10:46 -0500
From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
Subject: RE: [Histonet] TX Society meeting?
To: "Stephen Feher" <sfeher <@t> CMC-NH.ORG>,
<histonet <@t> lists.utsouthwestern.edu>, "Cheryl" <tkngflght <@t> yahoo.com>
Message-ID: <4BBD7365.5D38.00EF.0 <@t> swmail.sw.org>
Content-Type: text/plain; charset="us-ascii"
Website has all of the information
Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsearcy <@t> swmail.sw.org
254-724-2438
>>> "Feher, Stephen" <sfeher <@t> CMC-NH.ORG> 4/7/2010 4:13 PM >>>
Contact KDWYER3322 <@t> AOL.COM. April 23-25, Houston, TX. Be sure to sign up for the "Designing a LEAN Pathology Lab from Scratch" workshop on Saturday, April 24.
Steve
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, April 06, 2010 5:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] TX Society meeting?
Any word on Texas' regional histology meeting? I'm looking for dates and the registration info for my team--
Thanks!
Cheryl Kerry, HT(ASCP)
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TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:NSEARCY <@t> swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
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TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:NSEARCY <@t> swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
END:VCARD
------------------------------
Message: 5
Date: Thu, 8 Apr 2010 09:05:38 -0400
From: John PJ Coleman <jcolclefa <@t> aol.com>
Subject: [Histonet] trich/bouin's/FS/Inst cleaning/mast cells
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <90AB433A-AD51-4D0D-9CCC-DE8EE9E50B34 <@t> aol.com>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
Trichrome: Our Masson Trichrome procedure uses a combined
phosphotungstic/phosphomolybdic acid step for 20 minutes @ RT without
a rinse after, our smooth muscle stays RED..
Bouin's: Working in a rat research lab for years, we fixed all tissue
in bouins for 24 hours, rinsed in water for about an hour and stored
in 70% alcohol till processing. Is all your tissue post fixed in
bouins or just selected items? Why not Bouin's as a primary fixative?
We got great results on PTAH, Trichrome, Luxol Fast Blue stains.
Frozens: CAP says monitor FS times, if 90% are not within 20 minutes,
document the evaluation of why. ANP.11820
Instrument cleaning: We use TBQ which is virucidal and does not rust
instruments like bleach does.
Mast cells: We have not had unintentional mast cell staining on our
fleet of Ultras using the either Ultraview or the alk phos kits.
John PJ Coleman, HT(ASCP)QIHC
------------------------------
Message: 6
Date: Thu, 8 Apr 2010 09:54:02 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] eProtocols
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164015D4256F6 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
Hello Everyone,
Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS?
Thanks much!, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Confidentiality Notice:
This email, including any attachments is the
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Message: 7
Date: Thu, 8 Apr 2010 07:59:13 -0700 (PDT)
From: Bernard Martin <bernardgerard <@t> yahoo.com>
Subject: [Histonet] Sestrin 2
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <226491.17228.qm <@t> web37901.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi all,
I asked this a couple weeks ago, but thought I'd give it one more try-
has anyone had any experience with Sestrin 2 for IHC?
I can't find anything in the literature, and thought maybe some has tried it. There are ones available, but they all use the same picture, and I am concerned perhaps it doesn't work well. Funds are limited so we would like to get one that someone has had some success with. Thanks for any info you could provide!
Bern
------------------------------
Message: 8
Date: Thu, 8 Apr 2010 11:05:47 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] eProtocols
To: "Weems, Joyce" <JWeems <@t> sjha.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFEB57470A.4D497F9D-ON852576FF.005285FA-852576FF.0052ED7F <@t> chs.net>
Content-Type: text/plain; charset="US-ASCII"
I have printed off the CAP worksheets off the web site. We use them as
work sheets for the pathologist. He checks off and fills in what is
pertinent to the case, then the transcriptionist transcribes the Synoptic
Report into the final report directly under the Final Diagnosis.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
"Weems, Joyce" <JWeems <@t> sjha.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/08/2010 09:54 AM
To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] eProtocols
Hello Everyone,
Are any of your pathologists using the Cancer eProtocols provided by CAP?
If so you would you let me know of your experiences and how you interfaced
it with your LIS?
Thanks much!, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Confidentiality Notice:
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for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please reply to the
sender that you have received the message in
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Message: 9
Date: Thu, 8 Apr 2010 10:17:09 -0500
From: Cheri Miller <cmiller <@t> physlab.com>
Subject: [Histonet] RE: Traveling Histo Tech
To: "Marshall, Kimberly" <Kimberly.Marshall <@t> ahss.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E3C81A010935EA41B379AC765103F3BF31B3728F2E <@t> olsrv12>
Content-Type: text/plain; charset="us-ascii"
Only to say I am envious, good luck and have fun!
Cheryl Miller HT ASCP cm
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
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Message: 10
Date: Thu, 8 Apr 2010 16:33:01 +0100
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] RE: Travelling Histo Tech
To: 'Cheri Miller' <cmiller <@t> physlab.com>, "Marshall, Kimberly"
<Kimberly.Marshall <@t> ahss.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7722595275A4DD4FA225B92CDBF174A1E8CFFB6D32 <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"
So you could sum it up thus:- "Have knife, will slice for a price".
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: 08 April 2010 16:17
To: Marshall, Kimberly; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Traveling Histo Tech
Only to say I am envious, good luck and have fun!
Cheryl Miller HT ASCP cm
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
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Message: 11
Date: Thu, 8 Apr 2010 16:38:03 +0100
From: "Thotakura, Anil Kumar" <a.thotakura <@t> imperial.ac.uk>
Subject: [Histonet] <no subject>
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C7E3B6EB.49C5%a.thotakura <@t> imperial.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Hi All,
I have a problem with immunohistochmesitry. I am have raised my own antibody
and it is not commercially available. This monoclonal antibody works fine
for western blot. When it come to immunohistochemistry on mice liver frozen
section, it is binding every where. I can see staining in my control as
well.
Any suggestion or guess's from you guys?
Anil
------------------------------
Message: 12
Date: Thu, 8 Apr 2010 17:38:40 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: [Histonet] CD 25 protocol on Benchmark
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B5211D21B02545ECBFA6FB69A95BC282 <@t> dielangs.at>
Content-Type: text/plain; charset="us-ascii"
Hi Benchmark-users!
Can someone of you provide me with a protocol for CD 25 for FFPE tissue. We
have purchased the antibody from Cell marque and have found nuclear staining
in trephine bone marrow biopsy with mastocytosis. This specimen was
decalcified with formic acid.
Used protocol: CC1 mild, 1:10 for 40 min
I would be happy about any help.
Gudrun Lang
------------------------------
Message: 13
Date: Thu, 8 Apr 2010 17:27:26 +0100
From: julia hough <julia.m.hough <@t> hotmail.co.uk>
Subject: [Histonet] CD138 IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY137-W11C28946633CE4BF3CD888F9160 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Dear all.
Does anyone have a protocol for the immunohistochemistry staining of CD138 on paraffin embedded mouse tibia, including the fixation and decalcification protocol?
Thanks
BW
Julia
_________________________________________________________________
http://clk.atdmt.com/UKM/go/197222280/direct/01/
Do you have a story that started on Hotmail? Tell us now
------------------------------
Message: 14
Date: Thu, 8 Apr 2010 11:30:58 -0500
From: Bill <bill501 <@t> mindspring.com>
Subject: Re: [Histonet] eProtocols
To: "Weems, Joyce" <JWeems <@t> sjha.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <p0624080ec7e3b4a3c5bd@[4.244.150.137]>
Content-Type: text/plain; charset="us-ascii"
At 9:54 AM -0400 4/8/10, Weems, Joyce wrote:
>Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS?<
We export data from our LIS and pick it up in Word merge templates. We then use autotext to add the appropriate protocol info (modified) for the diagnosis.
Bill
------------------------------
Message: 15
Date: Thu, 08 Apr 2010 12:47:14 -0400
From: "Michael LaFriniere" <MLafrini <@t> csmlab.com>
Subject: Re: [Histonet] Just three months?????
To: <histonet <@t> lists.utsouthwestern.edu>, "Joe Nocito"
<jnocito <@t> satx.rr.com>, "Jeffrey Silverman" <pathmaster <@t> yahoo.com>
Message-ID: <4BBDD046.588C.00AF.0 <@t> csmlab.com>
Content-Type: text/plain; charset=US-ASCII
FYI
The Grandfather clause still stands as it always had....
Michael R. LaFriniere
Executive Director
Cytology Services of Maryland (CSM)
301-206-2555 ext 27 301-206-2595 fax
michael.lafriniere <@t> csmlab.com
>>> On 4/1/2010 at 8:46:10 PM, "Joe Nocito" <jnocito <@t> satx.rr.com> wrote:
I see that now, wasn't the first time I was wrong today, probably wouldn't
be the last either
----- Original Message -----
From: "Jeffrey Silverman" <pathmaster <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, March 31, 2010 5:03 PM
Subject: [Histonet] Just three months?????
CAP is now saying no more gross processing of small things that are entirely
submitted- it's all gross examination now whether we mean straining
currettings into a cassette or dissecting a complex cancer resection. Dumb
as all get out if you ask me.
As for the three month training thing, Joe, I'm not so sure about that. They
seem to spell out specific amounts of college education required IN ADDITION
to training in the laboratory.
The requirement first spells out two education choices-
1: an earned associates degree in medical laboratory science
OR 2:Education/training equivalent to the above that includes at least 60
semester
hours or equivalent from an accredited institution. This education
MUST (caps mine) include 24 semester hours of medical laboratory technology
courses, OR 24
semester hours of science courses that includes 6 semester hours of
chemistry,
6 semester hours of biology, and 12 semester hours of chemistry,
biology
or medical laboratory technology in any combination.
So in addition to all the college courses, which we all know you need to
count and measure six endoscopic biopsies and put them in a cassette, much
more training and experience needed than what an unregistered on the job
tech needs to orient and embed them properly LOL!!! CLIA then requires that
the
individual must have (additional) laboratory training including either
completion of
a clinical laboratory training program approved or accredited by
the ABHES, NAACLA, or other organization approved by HHS (note that
this training may be included in the 60 semester hours listed
above),
OR at least 3 months documented laboratory training in each
specialty in which the individual performs high complexity testing.
Now there are grandfathering clauses in CLIA which may enable folks (like
myself) to continue to gross. I haven't digested that yet, but I'm ready to
get that job in Pathmark if necessary.
Sheesh, does it ever end?
Jeff Silverman
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End of Histonet Digest, Vol 77, Issue 10
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