[Histonet] herring sperm dna

[Nicole Collette]

Hi, Emily,

We use Salmon Testes DNA for our hyb buffer for whole mount RNA in 
situ on mouse and frog embryos, Sigma catalog # D-9156, comes in 5 x 
1ml (11.0 mg/ml) tubes.  It says "for hybridization" on the package 
;) Hope that helps.

Sincerely,
Nicole Collette
Lawrence Livermore National Lab/UC Berkeley


At 12:10 PM -0400 4/7/10, Emily Sours wrote:
>Hello all!
>
>Roche has decided to stop manufacturing herring sperm DNA.  I thought this
>would be an easy product to replace but apparently there's roughly ten
>bjillion versions of herring sperm DNA.  I have no idea whether ours was
>sheared, sonicated, molecular biology grade, etc.  It just said herring
>sperm DNA on the label.  Any suggestions on a replacement? We are using
>this, btw, in hybridization buffer for RNA in situ  on chick embyro sections
>on slides.
>I called Roche and asked tech services what their replacement would be, but
>they just said, here's another DNA product which uses the term hybridization
>in its protocol, COT Human DNA, read the tech sheet to see if that's a good
>replacement.  Of course, the tech sheet says nothing about whether it would
>be good in in situ hybridization buffer, just that it's good for suppressing
>cross hybridization in DNA microarrays.  The really frustrating thing is
>their DIG in situ hybridization protocol calls for herring sperm DNA.
>
>Emily
>
>Shall we always be content with the ancient tinned salad of the subsidized
>novel? Or the tired ice-cream of poems which cry themselves to sleep in the
>refrigerators of the mind?
>-Lawrence Durrell, Clea
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