[Histonet] picro sirius red, sirius red connective tissue stain[Scanned]

[Itai Moshe]

picro Sirius red and Fast green protocol:

Sirius red (Sirius Red F3B)- 0.1% in Picric acid.
Fast Green - 0.03% in Picric acid (100%).
Can be re-used, filter twice before each use.


1) Deparaffinization and Rehydration:
A) Xylen - 10 min X2
B) Ethanol 100% - 2 Min X2
C) Ethanol 96% - 2 Min X1
D) Ethanol 80% - 2 Min X1
E) Ethanol 70% - 2 Min X1

2) Rinse in double distilled water (DDW) - 5 Min

3) Rinse in filtered Fast green - 20 Min

4) Wash with DDW ( just fill the slide box with DDW and empty).

5) Rinse in filtered Sirius red - 5 Min.

6) Wash with DDW ( just fill the slides box with DDW and empty).

7) Check in microscope, if the colors are not strong enough, repeat from
step 2 to 7.

That's it.

-- 
Itai Moshe
Mark Pines lab
Institute of Animal Sciences,Volcani Center.
Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew
University of Jerusalem
Israel

------Original Message-----
*Adam Bazama* baza0013 <@t> d.umn.edu
<histonet%40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20sirius%20red%20connective%20tissue%20stain%5BScanned%5D&In-Reply-To=>
*Mon Oct 6 10:27:37 CDT 2008*


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------------------------------

Hi Dave,



I noticed that you posted a request in march of 2004 on histonet for a
Sirius red fast green staining protocol. If you do have a protocol, would
you mind sending it to me. I have had a hard time finding one to compare to
mine and my lack luster results.



Thank you,





Adam Bazama, B.S.

Junior Scientist

Lillehei Heart Institute Histology and Microscopy Core Facility

University of Minnesota Medical School, Division of Cardiology
4-266 Nils Hasselmo Hall
312 Church Street SE
Minneapolis, MN  55455
Lab Desk: 612-625-6779
Cell: 952-334-0607

--------------------------------------------

*David Rushworth* David.Rushworth <@t> mail.bhrv.nwest.nhs.uk
<histonet%40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20sirius%20red%20connective%20tissue%20stain%5BScanned%5D&In-Reply-To=>
*Thu Mar 4 09:00:18 CST 2004*


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------------------------------

Can someone please supply method for Sirius red-fast green stain for
connective tissue.
Thanks in anticipation.
Dave.



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