[Histonet] new fluorescent dyes

Adam . anonwums1 <@t> gmail.com
Fri Apr 2 13:40:18 CDT 2010


I've tried Dylight 488, Dylight 594, and Dylight 649. They all seem to be
pretty bright and don't seem to photobleach much over the course of my
experiments. The longest I've looked for signal is about 2 months after the
slides were stored in a dark area, and they seem to retain most of their
brightness. I've never compared them to the AlexaFluors though, but I'm
pretty happy with them.

Adam

On Fri, Apr 2, 2010 at 1:35 PM, Karen Doty <k.fd <@t> live.com> wrote:

>
> Has anyone tried the Dylight fluorescent dyes? Are they significantly
> better than the Alexa Fluors? In what ways? I'd really appreciate some
> opinions before I set up a fluorescent dye project.
>
>
>
> Thanks very much,
>
> Karen Doty
>
>
>
> University of Illinois
>
> Dept. of Veterinary Biosciences
>
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