[Histonet] Assay for cell death needed

[John Kiernan]

Dead cells stain intensely with anionic dyes such as eosin. For nervous tissue there's a simple method using acid fuchsine and toluidine blue. Normal neurons are blue; dead and dying ones are red. 
See Auer RN et al 1984 Acta Neuropathologica 64: 177-191.
Also  Biotechnic & Histochemistry 73: 244-254 (1998).
The second paper includes an investigation of why the nuclei and cytoplasm of moribund cells stain red.
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Alexandra Meinl <alexandra.meinl <@t> gmail.com>
Date: Thursday, April 1, 2010 5:01
Subject: [Histonet] Assay for cell death needed
To: Histonet <histonet <@t> lists.utsouthwestern.edu>

> Hello Histonetters,
> 
> We desperately need an assay that detects cell death in human 
> FFPE sections
> (regardless if its apoptosis or necrosis).
> Some kind of kit (quick 'n easy) would be really fine!
> 
> Any suggestions?
> 
> Many thanks,
> 
> Alexandra
> 
> ************************************************
> Dr. Alexandra Meinl
> Ludwig Boltzmann Institute
> for Experimental and Clinical Traumatology
> Austrian Cluster for Tissue Regeneration
> Histology
> Donaueschingenstrasse 13
> 1200 Vienna - Austria
> 
> Contact @ Bernhard Gottlieb University School of Dentistry,
> Waehringerstr. 25a, A-1090 Vienna
> tel:  +43 1 4277 67026
> fax: +43 1 4277 67019
> email: alexandra.meinl <@t> trauma.lbg.ac.at
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