[Histonet] RE: Histonet Digest, Vol 70, Issue 35

Mon Sep 28 07:44:47 CDT 2009

I have used the ThermoFisher Excelsior at two different facilities. It is a wonderful processor and maintenance is a breeze, and uses less alcohol and xylene. Please take time to look at it. 

Elaine Monroe, Lead Histotech

Spring Memorial Hospital

> From: histonet-request <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 70, Issue 35
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Sun, 27 Sep 2009 10:00:31 -0700
> 
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> Today's Topics:
> 
> 1. MSI Testing (Gomez, Milton)
> 2. Processors (Rebecca Johnson)
> 3. Going from immmunohistochemistry to immunofluorescence (Adam .)
> 4. Re: Processors (Rene J Buesa)
> 5. Laura Miller is Out of the Office.
> (Laura.Miller <@t> leica-microsystems.com)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Sat, 26 Sep 2009 16:33:58 -0600
> From: "Gomez, Milton" <milton.gomez <@t> aruplab.com>
> Subject: [Histonet] MSI Testing
> To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <6995F66A2196514DB0C4CAA5A32EA2931066F5 <@t> postoffice01.aruplab.net>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Dr. Richard Cartun,
> 
> Our laboratory offers MSI testing and typically do go thru a Genetic Counselor first.
> 
> Thanks,
> 
> Milton A. Gomez, HTL (ASCP)
> Technical Supervisor
> Immunohistochemistry Department
> ARUP Laboratories, Inc.
> 500 Chipeta Way
> Salt Lake City, UT 84108-1221
> Desk Phone: 801-583-2787, ext.3869
> Lab. Phone: 801-584-5257/5242
> Fax: 801-584-5217
> E-mail: milton.gomez <@t> aruplab.com <mailto:milton.gomez <@t> aruplab.com> 
> Web: www.aruplab.com <http://www.aruplab.com/> 
> 
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> 
> ------------------------------
> 
> Message: 2
> Date: Sat, 26 Sep 2009 18:31:28 -0500
> From: "Rebecca Johnson" <raj <@t> bluemarble.net>
> Subject: [Histonet] Processors
> To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <2B62291D4807407A8FE740A5BEC8D4D6 <@t> CHURCH>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
> 
> I need advice from you wonderful techs about what processor would be best 
> for a Derm Lab. I am going to the NSH next week and with your advice I will 
> be able to look at some. I work now in a hospital and we have 2 VIP (Sakura) 
> processors. I like them very much. But for a derm lab would some of the 
> express processors be better. Thank for all your help.
> Becky 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Sat, 26 Sep 2009 18:55:07 -0500
> From: "Adam ." <anonwums1 <@t> gmail.com>
> Subject: [Histonet] Going from immmunohistochemistry to
> immunofluorescence
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <858249120909261655x63344883ie135fdd6a5c54b22 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Hi all,
> 
> I have a few antibodies that I have successfully gotten to work using
> immunohistochemistry (primary + biotinylated secondary + SA-HRP + DAB). I am
> hoping to move from IHC to immunofluorescence. I tried the same staining
> using either a fluorescently conjugated secondary or a biotinylated
> secondary and an avidin conjugated fluorophore, but I don't see any staining
> at all. I understand the peroxidase chemistry is a lot more sensitive than
> fluorescence, so my question is, if I can get IHC to work, should I be able
> to get IF to work? If so, how would I go about troubleshooting it? How
> important is the flourophore (i.e. Alexa conjugates vs Texas Red vs
> Rhodamine).
> 
> Yes, I know I could do dual immunohistochemistry, but I like fluorescence
> since you can see each stain independently and then merge them
> electronically. If anyone has a great suggestion on some chromagens that
> work great together, I would welcome those suggestions as well.
> 
> Thanks,
> Adam
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Sun, 27 Sep 2009 05:50:43 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Processors
> To: histonet <Histonet <@t> lists.utsouthwestern.edu>, Rebecca Johnson
> <raj <@t> bluemarble.net>
> Message-ID: <545739.43168.qm <@t> web65716.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> For any lab, not only for derm, take a look at the Skura instruments.
> René J.
> 
> --- On Sat, 9/26/09, Rebecca Johnson <raj <@t> bluemarble.net> wrote:
> 
> 
> From: Rebecca Johnson <raj <@t> bluemarble.net>
> Subject: [Histonet] Processors
> To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Saturday, September 26, 2009, 7:31 PM
> 
> 
> I need advice from you wonderful techs about what processor would be best for a Derm Lab.  I am going to the NSH next week and with your advice I will be able to look at some. I work now in a hospital and we have 2 VIP (Sakura) processors. I like them very much. But for a derm lab would some of the express processors be better.  Thank for all your help.
> Becky 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Sun, 27 Sep 2009 09:10:15 -0500
> From: Laura.Miller <@t> leica-microsystems.com
> Subject: [Histonet] Laura Miller is Out of the Office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFA8A5A30A.5FDB3C21-ON8625763E.004DD7ED-8625763E.004DD7ED <@t> leica-microsystems.com>
> 
> Content-Type: text/plain; charset=US-ASCII
> 
> 
> I will be out of the office starting 09/25/2009 and will not return until
> 09/29/2009.
> 
> I will respond to your email on Tuesday. Thanks!
> 
> 
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> End of Histonet Digest, Vol 70, Issue 35
> ****************************************

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