[Histonet] Re: Granular appearance to chromagen

Sherwood, Margaret MSHERWOOD <@t> PARTNERS.ORG
Fri Sep 25 15:51:00 CDT 2009

Has anyone experienced a granular appearance to their chromagen pattern?  I just
did a run with Vector's NovaRed and all the slides show "staining" but on higher
power, the appearance of the chromagen is granular (like it broke down?)  

I never had this problem with DAB and this is the second time I have experienced
it with NovaRed.  I use their kit, so after the addition of each component, I
vortex to make sure it is well-mixed.  Could this be the problem?

Please advise.


Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 
msherwood <@t> partners.org 

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