[Histonet] Steiner and Steiner

[Tony Henwood]

Nikki,

The sensitisation step, from my experience, improves the silver
deposition on the bacteria and decreases background staining. Margeson &
Chappman (1996 Use of Zinc Formalin as a Sensitizer in Silver Stains for
Spirochetes J Histotechnol 19(2):135-138) substituted zinc formalin for
uranyl nitrate in the Steiner techniques and found the resulting
spirochete staining to be as good as or better than when uranyl nitrate
was used.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wahlberg, Nikki
Sent: Friday, 25 September 2009 3:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Steiner and Steiner


Does anyone have a protocol for the Steiner and Steiner stain that does
not use Uranyl Nitrate?  Our EHS department will not allow us to order
this chemical anymore because it is too expensive to dispose of.  If
anyone has any methods of disposal that are not too pricey I would be
interested in them as well.
 
Thank you very much,
Nikki
 
Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com

CONFIDENTIALITY NOTICE:  This e-mail transmission may contain
confidential or legally privileged information that is intended only for
the individual or entity named in the e-mail address.  Any unauthorized
distribution or copying of this transmittal or its attachments, if any,
is prohibited.  If you have received this e-mail transmission in error,
please reply to the sender, so that Boston Scientific Corporation can
arrange for proper delivery, then please delete the message from your
inbox.

 

 
 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************