[Histonet] Steiner and Steiner

Tony Henwood AnthonyH <@t> chw.edu.au
Thu Sep 24 18:11:00 CDT 2009


The sensitisation step, from my experience, improves the silver
deposition on the bacteria and decreases background staining. Margeson &
Chappman (1996 Use of Zinc Formalin as a Sensitizer in Silver Stains for
Spirochetes J Histotechnol 19(2):135-138) substituted zinc formalin for
uranyl nitrate in the Steiner techniques and found the resulting
spirochete staining to be as good as or better than when uranyl nitrate
was used.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wahlberg, Nikki
Sent: Friday, 25 September 2009 3:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Steiner and Steiner

Does anyone have a protocol for the Steiner and Steiner stain that does
not use Uranyl Nitrate?  Our EHS department will not allow us to order
this chemical anymore because it is too expensive to dispose of.  If
anyone has any methods of disposal that are not too pricey I would be
interested in them as well.
Thank you very much,
Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
wahlbern <@t> bsci.com

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