[Histonet] CD31 Mouse Liver Staining
JEFFREY S HARDING
jsharding <@t> wisc.edu
Thu Sep 24 13:46:16 CDT 2009
I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections.
I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal.
My question is regarding fixation methods IMMEDIATELY after dissection. Does anyone have a good summary of fixation methods prior to freezing and their effects on CD31 staining? Does the exact fixation even matter that much? Everyone seems to have something different.
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