[Histonet] CD31 Mouse Liver Staining

[JEFFREY S HARDING]

 Hello,

    I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections.   

   I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal. 

  My question is regarding fixation methods IMMEDIATELY after dissection.  Does anyone have a good summary of fixation methods prior to freezing and their effects on CD31 staining?  Does the exact fixation even matter that much?  Everyone seems to have something different.   

Thanks!
Jeff