[Histonet] f4/80 on wax embedded mouse tibia sections

[julia hough]

Hello Histonetters.

 

I have recently stained for f4/80 macrophage marker on wax embedded mouse
tibia sections. I have managed to get staining however, I also have staining
on my negative control, which seems quite specific. 

I have investigated this and there seems to be no problems with the DAB and
all reagents used are exactly the same down to where they are bought as
those recommended in a protocol.

 

If you have managed to stain for f4/80 on tibia sections without getting
staining on your control or you have any explanations for this I would much
appreciate hearing from you to compare protocols. 

 

Thanks

 

Julia