[Histonet] mib-1

[godsgalnow <@t> aol.com]

Correct....the formalin is neglegable.? We have been using this antobody for years with minor issues.? Over the past few weeks we are running into a problem with false negatives.? The control is working fine.


-----Original Message-----
From: Greg Dobbin <gvdobbin <@t> ihis.org>
To: godsgalnow <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Sent: Thu, Sep 17, 2009 8:09 am
Subject: Re: [Histonet] mib-1



"Alcohol based" suggests to me an absence of formalin. In which case,
epitope retreival is not indicated.
Also, you didn't say if it had been working and stopped or if this was
a new marker for your lab.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> <godsgalnow <@t> aol.com> 9/16/2009 3:44 PM >>>

I have to turn to the experts here.? I am having problems with the
MIB-1.? I am having an issue with the patient samples working.? We use
an alcohol based fixative....it has always worked until recently.



I have tried everything I can think of to no avail.? I have decreased
the titer, increased the incubation, tried a different retrieval
solution, tried a different detection...nothing is working.



Anyone else having any issues?
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