[Histonet] Peloris tissue processor

Stella Argyriou Stella.Argyriou <@t> leica-microsystems.com
Wed Sep 16 20:30:46 CDT 2009


Dr. Evette Demaisip,
Thank you for your posting.
We have forwarded this to your Leica representative for the Philippines.
They should be in contact with you shortly.
Alternatively in future you may also contact histosupport <@t> leica-microsystems.com for further assistance.

Best Regards,


Stella Argyriou
Global Product Manager

Biosystems Division
Leica Biosystems Melbourne Pty Ltd
495 Blackburn Road
Mt Waverley VIC 3149
Australia
Telephone +61 3 9211 7514
Facsimile  +61 3 9211 7401

www.leica-microsystems.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of evette demaisip
Sent: Thursday, 10 September 2009 10:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Peloris tissue processor

Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue processor and have been test processing tissues under different hour protocols. We encountered problems in the 8-hr and 5-hr protocols.Both produced tissues that were too difficult to section in the microtome.  My histotechs showed me tissues that were like brittle plastics. Despite reprocessing, the tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were too thickly sampled and poorly fixed. May ask for feedback/advice on the ff;?
1. Possible reasons?
2. Steps to prevent this in the future?
3. Reprocessing protocol in Peloris.

Thank you so much.
Dr. Evette Demaisip
Pathologist,Philippines


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