[Histonet] Re: ImmPACT DAB kit

Hobbs, Carl carl.hobbs <@t> kcl.ac.uk
Wed Sep 16 13:09:39 CDT 2009

Many thanks for the replies regarding this reagent.
I delayed purchasing this as I was very skeptical: however,  I can achieve a 1/5-10 fold dilution factor for my precious low affinity (? thus low dilution factor) Abs.
I will not be using ImmPACT chromogen kit routinely as, for the vast majority of Abs, I do not find it neccessary.
I have been informed ( courtesy of Histonetters who replied) that Dako offer a similarly sensitive kit.
For the record, I routinely use a std manual  stABCpx-DAB (DAB  in-house prepared)detection system in a Research Lab with very little "clinical" time-constraints)

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