[Histonet] mice legs

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Tue Sep 15 10:13:59 CDT 2009

I did a lot of  IHC work with whole mouse legs where we had to pay close 
attention to the tibia, as well as the joint.    When the mice were 
necropsied, I had the prosectors remove all the soft tissue and foot 
without disrupting the joint.  They could do this easily with fresh tissue 
as opposed to after fixation.   The leg bones were fixed for no more than 
48 hours, and decalcified in home-brewed 5% formic acid overnight on a 
shaker table.   I then trimmed them to expose the joint and bone marrow 
prior to processing on a routine program, about 45 minutes per station. 
Perfect ever single time.  IHC was beautiful. 

Hope this helps.

Jackie O'

"Shaw, Sharon" <shshaw <@t> WPI.EDU>
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
09/15/2009 08:46 AM
[Histonet] mice legs
Sent by:
histonet-bounces <@t> lists.utsouthwestern.edu

Good Morning Histo World,

I would like to know if anyone is working with mice legs, I have a PI that 
I work with that wants to process the whole leg, the problem is I need to 
decal it first and is wondering if the decal will break down the tissue, I 
think it would he doesn't think so. And if anyone has do this would it be 
possible to share your protocol with me from decal to processing.

Sharon- WPI
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