[Histonet] mercury vs led lights

[Morken, Tim]

Merced wrote:  " I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 
(far-red) fluorophores, but they cannot make them in the red (Alexa Fluor 
555, Cy3, TRITC, etc.) This is per our Zeiss rep."

 

I'm not sure why the Zeiss rep said that. Maybe Zeiss itself does not carry the LED's in that range but I have used up to 655nm LED. 

See this for details on other LED's for fluorescence work:

http://www.fraensrl.com/images/fraen_fluorochromes.pdf



Tim Morken
UCSF Medical Center
San Francisco, CA  
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M Leiker
Sent: Wednesday, September 09, 2009 10:44 AM
To: Nejat Yilmaz; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] mercury vs led lights

I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 
(far-red) fluorophores, but they cannot make them in the red (Alexa Fluor 
555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted 
microscope we have LEDs and we also have a mercury lamp to cover the red 
range. The Zeiss switches nicely and very smoothly between the light 
sources during viewing and image acquisition.

Sorry for the highly untechnical description, but that is all I know about 
it.  :-)

Regards,
Merced
--On Wednesday, September 09, 2009 12:21 PM -0400 Merced M Leiker 
<leiker <@t> buffalo.edu> wrote:

> LEDs don't quench your fluorescence as quickly as mercury.
>
>
> --On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz
> <nyilmaz <@t> mersin.edu.tr> wrote:
>
>> Dear Colleagues,
>>
>> We're attempted to buy a new inverted fluorescence microscope for our
>> research lab. Distrubitor of leica offered us a new type system working
>> with led lights which is about same price. Does anybody know advantages
>> and disadvantages of this system compared to conventional (with mercury
>> lamp) fluorescence microscopes.
>> Thanks in advance.
>>
>> Necat Yilmaz
>>
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>
>
>
> Merced M Leiker
> Research Technician II
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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