[Histonet] CD33 antibody

[Justin Peters]

I am having some trouble getting good staining with the CD33 antibody
from Novocastra.  I am trying to stain FFPE bone marrow biopsies
decalcified in Formical for 1 hour prior to processing.  The staining is
very weak even at a 1:25 dilution using a pH 9.0 antigen retrieval
solution (recommended) in a pressure cooker.  Has anyone had any luck
with this antibody on bone marrow?  I have tried this protocol with the
blood clots sent with the bone marrow biopsies and it works just fine so
I am guessing that the decal is affecting the staining.  Any thoughts??

 

Justin Peters, HTL (ASCP)

IHC Supervisor

Bostwick Laboratories(tm)
For Absolute Confidence(r)

4355 Innslake Drive
Glen Allen, Virginia 23060
Phone:    (804) 967-9225 ext. 1831
Cell:        (804) 822-6084
Email: jpeters <@t> bostwicklaboratories.com
<mailto:jpeters <@t> bostwicklaboratories.com>