[Histonet] Re: anti-GFP antibody

Adam . anonwums1 <@t> gmail.com
Wed Sep 2 16:05:03 CDT 2009

Hi all,

Just to update you, I ordered Abcam's ab13970 chicken anti-GFP and tested it
out in paraffin embedded sections with HIER for 10 minutes in Dako target
retrieval system. I came in with a Dylight 488 secondary from Jackson
Immunoresearch at 1 ug / mL. It worked beautifully at 1:500, 1:1000, and
1:2000. I should've titered it even further down.

Thanks for all your help,

On Thu, Aug 20, 2009 at 1:38 PM, Hobbs, Carl <carl.hobbs <@t> kcl.ac.uk> wrote:

> I am sure someone has a cunning combination!
>  I use Abcam's ab13970 chicken anti  GFP in Pwax sections after HIER.
> For me, it is very good
> Image at Abcam or here : http://www.immunoportal.com/index.php
> Good luck!
> carl
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