[Histonet] new antibody validations

[Campbell, Sharon]

Hello Histonet!
We are going to be optimizing and validating new antibodies. What is the recommended number of cases for validating once we have optimized? I have heard 20 and also 40 cases. Also, How is a negative control to be optimized and validated?
Thank you.

Sharon Campbell, HTL(ASCP)CM, BSBM
Histology Supervisor
Celligent Diagnostics, LLC
101 East W.T. Harris Blvd, Suite 1212
Charlotte, NC  28262
800-524-6779 ext. 104
704-970-3304 Direct Line
scampbell <@t> celligent.net