[Histonet] imaging organ cultures

[Alice Fleming]

Hi,
I am doing whole mount IHCs on embryonic organ cultures and imaging them on a confocal microscope.  The IHCs are going well, but I’m having trouble making the tissue stay in a specific position for imaging.  I have been putting the tissue in the wells of depression slides, adding about 30uL of Vectashield mounting medium, then coverslipping.  This works fine, except that I have little control over the final position of the tissue—as I put the coverslip down, the tissue often moves.
Any suggestions would be welcome!  Is there, for example, some kind of optically neutral agarose that I could spot around the tissue before adding the Vectashield and coverslip??  Or—other ideas??
Thanks,
Alice

Alice Fleming
Department of Human Genetics
University of California Los Angeles
Los Angeles, CA 90095
310-267-2456





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