[Histonet] Green Fluorescent Protein

[Merced M Leiker]

The fluorescence itself will not be detectable, you would have to use an 
indirect means (like antibody, which you mentioned). That's just from my 
experience with it.  Maybe Gayle Callis has a suggestion...

Regards,

Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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--On Tuesday, October 27, 2009 2:48 PM +0200 Melanie Black 
<Melanie.Black <@t> uct.ac.za> wrote:

> Hi
>
> I am looking to demonstrate Green fluorescent protein in Para
> formaldehyde fixed, processed rat tissue. Apart from using an antibody
> against GFP, can the native GFP be detected? Gayle Callis, I believe you
> may be able to help me with this method.
>
> Thanks
> Melanie
>
> Melanie Black
> 082 469 3352
>
> Cardiovascular Research Unit
> 3rd Floor; Chris Barnard Building
> Medical School;
> Observatory. 7925.
> University of Cape Town.
> South Africa.
>
>
>
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.