[Histonet] Xylene Substitute

[Rene J Buesa]

Sheila:
1- For tissue processing use isopropanol mixed with mineral oil.
2- For dewaxing the sections before staining use Dish Washing soap.
3- To prepare the slides before coverslipping don't use anything, just oven dry the sections and cover.
Under separate cover I am sending the detailed procedures.
René J.

--- On Fri, 10/23/09, Sheila Haas <micropathlabs <@t> yahoo.com> wrote:


From: Sheila Haas <micropathlabs <@t> yahoo.com>
Subject: [Histonet] Xylene Substitute
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, October 23, 2009, 3:07 PM


Hi all. Could someone recommend a xylene substitute and mounting media to use for staining and coverslipping (no vendors please)? I am considering processing without xylene and would like to discontinue everywhere if possible. My previous experience was a lifetime ago and I'm sure things have advanced since then.
Thank you.
 
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



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